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| Product Name | Oil Red O Lipid Staining Kit for Cell Culture |
| Catalog No. | ATRSA-0022 |
| Description | A histochemical staining kit for visualizing neutral lipid droplets and adipocyte differentiation in cultured cells. Oil Red O is a fat-soluble diazo dye that partitions into lipid droplets, producing an intense red-orange stain against a hematoxylin-counterstained background. The kit is optimized for adherent cell monolayers and provides quantitative extraction capability for spectrophotometric lipid content measurement. |
| Intended Use | Visualization and quantification of neutral lipid accumulation in cultured cells for adipogenesis and steatosis studies. |
| Principle / Technology | Oil Red O dye partitioning into neutral lipid droplets by hydrophobic interaction |
| Detection Method | Light microscopy (brightfield) for imaging; spectrophotometry at 510 nm for extraction quantification |
| Sample Type | Adherent cultured cells including 3T3-L1 adipocytes, HepG2 hepatocytes, and primary adipocyte cultures |
| Performance Range / Specifications | Staining intensity linear with triglyceride content from 0.01–1.0 absorbance units at 510 nm |
| Sensitivity / LOD | Detects lipid droplets as small as 0.5 µm in differentiated cells |
| Specificity | Selectively stains neutral lipids (triglycerides, cholesteryl esters); does not stain phospholipid membranes |
| Reaction Conditions / Protocol | Wash cells with PBS; fix with 10% formalin 30 min; rinse with 60% isopropanol; stain with Oil Red O working solution 15 min; rinse; counterstain with hematoxylin 1 min; mount with aqueous medium; For extraction: add 100% isopropanol 10 min, read at 510 nm |
| Components / Formulation | Oil Red O stock solution, hematoxylin counterstain, isopropanol, formalin fixative, PBS |
| Storage Conditions | Oil Red O: room temperature; hematoxylin: room temperature; formalin: room temperature |
| Shelf Life | 12 months for Oil Red O; 18 months for other reagents |
| Package Specifications | 100 staining assays (24-well plate format) |
| Product Form | Liquid reagents |
| Quality Control | Each lot validated by staining differentiated versus undifferentiated 3T3-L1 cells; staining intensity correlated with triglyceride content |
| Key Features | Extraction and spectrophotometric readout enables objective quantification complementing subjective microscopic assessment |
| Purity | Dye content as specified; filtered to remove particulates |
| Concentration | As specified; ready-to-use or concentrate format |
| Activity / Unit Definition | Staining intensity and specificity validated on reference tissue/cell preparations |
| Molecular Weight | Varies by dye component as specified in product documentation |
| Source / Origin | Synthetic dyes and biological stains; certified by Biological Stain Commission where applicable |
| pH Range / Optimal pH | pH 6.0–7.0 for Romanowsky-type stains; varies by formulation |
| Shipping Conditions | Ambient temperature; protect from light and moisture |
| Expiration Date / Stability | 24 months in unopened container; protect from light |
| Regulatory / Compliance | For in vitro diagnostic use in histology and cytology; CE-marked options available |
| Compatibility | Compatible with formalin-fixed tissue sections, blood smears, and cytological preparations |
| Recommended Buffer System | Use with provided buffer or phosphate buffer pH 6.8 as specified |
| Application Notes / Precautions | Avoid contact with skin and clothing; stains are permanent; use appropriate PPE |
| Batch-to-Batch Consistency | Staining intensity and differential contrast validated on reference specimens per lot |
For research use only, not for clinical use.
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