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| Product Name | Nucleic Acid Sample Diluent, TE-Based, Nuclease-Free |
| Catalog No. | ATRD-0021 |
| Description | Nuclease-free TE-based diluent (Tris-EDTA, pH 8.0) for dilution of DNA and RNA samples in molecular biology workflows. Filtered, autoclaved, and lot-tested for DNase, RNase, and nucleic acid contamination. EDTA chelates divalent cations required for nuclease activity, providing passive nuclease protection. |
| Intended Use | DNA/RNA dilution for PCR, qPCR, sequencing, microarrays; nucleic acid standard preparation; short-term diluted sample storage; oligonucleotide resuspension. |
| Principle / Technology | Tris maintains pH 8.0 for nucleic acid stability; EDTA chelates Mg²⁺/Ca²⁺ nuclease cofactors providing passive inhibition |
| Detection Method | Ready-to-use; no dilution required |
| Components / Formulation | Tris-HCl (10 mM, pH 8.0), EDTA (1 mM), nuclease-free water; 0.22 µm filtered, autoclaved |
| Storage Conditions | 15-25 °C; keep tightly closed to prevent nuclease contamination |
| Shelf Life | 24 months; use within 12 months after opening |
| Package Specifications | 50 mL, 100 mL, 500 mL, 1 L HDPE bottles |
| Product Form | Clear colorless liquid, sterile, ready-to-use |
| Key Features | Nuclease-free certified per lot; autoclaved and filtered; EDTA passive nuclease protection; endotoxin <0.01 EU/mL; DEPC-free formulation |
| Purity | Tris ≥99.9%; EDTA ≥99.5%; DNase/RNase: none detected |
| Concentration | Tris-HCl 10 mM ± 0.2 mM; EDTA 1 mM ± 0.05 mM |
| Activity / Unit Definition | Dilution linearity R² ≥0.99 across specified range |
| Molecular Weight | Not applicable — buffered protein solution |
| Source / Origin | Bovine serum albumin, casein, and synthetic buffer components |
| pH Range / Optimal pH | pH 8.0 ± 0.05 at 25 °C |
| Shipping Conditions | Ambient temperature |
| Expiration Date / Stability | 24 months at 15-25 °C; aliquot after opening; single-use aliquots recommended |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with ELISA, CLIA, and immunoturbidimetric assay platforms |
| Recommended Buffer System | PBS-based with protein stabilizers and antimicrobial preservative |
| Application Notes / Precautions | Bring to room temperature before use; avoid repeated freeze-thaw; use aseptic technique for opened bottles |
| Batch-to-Batch Consistency | Dilution recovery 90–110% for reference analyte panel per lot |
For research use only, not for clinical use.
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