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| Product Name | 0.4% Trypan Blue Solution |
| Catalog No. | RESK-0504 |
| Description | Trypan Blue is an azo, hydrophilic acidic blue dye. It can penetrate the cell membranes of dead and dying cells, staining them blue, while living cells, owing to the integrity of their cell membranes, exclude the dye. Based on this color change, viable cells (which appear transparent and colorless) can be distinguished from dead cells, and this principle is widely used for the routine assessment of cell viability levels. Trypan Blue can also stain collagen and amyloid. The complex formed by Trypan Blue binding to protein emits red fluorescence. In addition, Trypan Blue (at an appropriate concentration) is commonly used to quench autofluorescence or other fluorescent signals at the cell surface. This product is a 0.4% (w/v) Trypan Blue staining solution in physiological saline, supplied sterile and of cell culture grade. |
| Intended Use | Routine assessment of cell viability levels by dye exclusion; distinguishing viable cells (transparent and colorless) from dead cells (blue). Trypan Blue can also stain collagen and amyloid, and at an appropriate concentration it is commonly used to quench autofluorescence or other fluorescent signals at the cell surface. |
| Principle / Technology | Trypan Blue is an azo, hydrophilic acidic blue dye that penetrates the compromised cell membranes of dead and dying cells, staining them blue, while living cells with intact cell membranes exclude the dye. Viable and dead cells are thus distinguished by color. |
| Detection Method | Light microscopy: viable cells appear transparent and colorless, while dead cells are stained blue. |
| Sample Type | Cultured cells (cell suspensions). |
| Performance Range / Specifications | 0.4% (w/v) Trypan Blue in physiological saline; supplied sterile; cell culture grade. |
| Specificity | Selectively stains dead and dying cells with compromised cell membranes blue; living cells with intact membranes exclude the dye and remain colorless. |
| Reaction Conditions / Protocol | Mix the cell sample with the 0.4% Trypan Blue solution, then examine under a light microscope: viable cells are transparent and colorless, dead cells are blue. |
| Components / Formulation | 0.4% (w/v) Trypan Blue dissolved in physiological saline; sterile; cell culture grade. |
| Storage Conditions | Store at room temperature. |
| Shelf Life | 2 years when stored as directed. |
| Package Specifications | 20 mL 100 mL |
| Product Form | Liquid (sterile solution in physiological saline). |
| Concentration | 0.4% (w/v) |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for 2 years when stored at room temperature. |
| Regulatory / Compliance | For research use only. |
| Compatibility | At an appropriate concentration, Trypan Blue is commonly used to quench autofluorescence or other fluorescent signals at the cell surface. |
| Recommended Buffer System | Supplied in physiological saline. |
| Application Notes / Precautions | Trypan Blue can also stain collagen and amyloid; the complex formed with protein emits red fluorescence. |
For research use only, not for clinical use.
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