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| Product Name | Nitrotetrazolium Blue Chloride (NBT) |
| Catalog No. | RERE-0047 |
| Description | Nitrotetrazolium Blue chloride forms the classical NBT/BCIP detection system with 5-bromo-4-chloro-3-indolyl phosphate. Alkaline phosphatase first hydrolyses BCIP to a reactive intermediate that dimerises into an indigo dye, and the two reducing equivalents released by that dimerisation reduce NBT into an insoluble purple blue NBT formazan deposited exactly where the enzyme sits. The system needs no detection instrument and delivers sharply readable colour. Beyond enzyme histochemistry NBT also serves as a redox indicator for dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase and phosphofructokinase activity gels, and as a chromogenic indicator in blood bacterial infection testing. |
| Intended Use | Alkaline phosphatase based detection on Western, Northern and Southern blots, in situ hybridization, immunohistochemistry, alkaline phosphatase staining for iPS colony identification, enzyme activity gels and redox indicator assays. |
| Principle / Technology | Reducing equivalents from the BCIP enzymatic reaction reduce NBT to an insoluble purple blue formazan precipitate at the enzyme site. |
| Detection Method | Alkaline phosphatase labelled probes and antibodies; dehydrogenase activity in activity gels |
| Sample Type | Blot membranes, tissue and cell preparations, polyacrylamide activity gels |
| Performance Range / Specifications | Deep purple to blue purple deposition visible without instrumentation. |
| Specificity | Formazan precipitates only where enzymatic reducing activity is present. |
| Reaction Conditions / Protocol | Dissolve in water or methanol at 10 mg/mL as a stock and combine with BCIP in the working buffer before incubating the specimen. |
| Storage Conditions | Powder at 4 °C protected from light |
| Shelf Life | 3 years (powder) |
| Package Specifications | 1 g; 100 mg |
| Product Form | Yellow crystalline powder |
| Key Features | Water soluble at 10 mg/mL; forms the classical NBT/BCIP pair; doubles as a redox indicator across enzyme assays. |
| Molecular Weight | 817.6 |
| Shipping Conditions | Ambient temperature |
| Regulatory / Compliance | For research use. |
| Compatibility | Soluble in water (10 mg/mL) and methanol. |
| Batch-to-Batch Consistency | Lot specific certificate of analysis. |
For research use only, not for clinical use.
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