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| Product Name | MTT Solution, 5 mg/mL (CAS 298-93-1) |
| Catalog No. | RERE-0427 |
| Description | MTT, the yellow tetrazolium salt known formally as thiazolyl blue, turns purple wherever metabolically active cells reduce it, forming crystalline formazan that dissolves for absorbance reading. That single property has made it one of the most cited reagents in proliferation and cytotoxicity testing. This ready-to-use 5 mg/mL stock in PBS spares the laboratory the weighing and dissolving of a reagent that is notoriously slow to prepare, and the frozen, light-protected vial keeps it potent. |
| Intended Use | Colorimetric detection of cell proliferation and cytotoxicity in microplate and dish formats. |
| Principle / Technology | Active cellular metabolism reduces the tetrazolium ring to insoluble purple formazan crystals. |
| Specificity | 5 mg/mL MTT in PBS; CAS 298-93-1. |
| Reaction Conditions / Protocol | Add to cultures, incubate until formazan develops, solubilise the crystals and read absorbance. |
| Storage Conditions | -20°C; protected from light; 12 months. |
| Package Specifications | 5 mL, 10 mL |
| Key Features | Pre-dissolved convenient strength; PBS-based; skips the slow preparation of powder MTT. |
For research use only, not for clinical use.
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