- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | MTT Cell Viability and Proliferation Assay Kit |
| Catalog No. | CATR-HMM-0035 |
| Description | A tetrazolium dye-based colorimetric kit designed to assess cellular metabolic activity as an indicator of cell viability, proliferation, and cytotoxicity. The yellow MTT reagent is reduced by mitochondrial dehydrogenases in metabolically active cells to form insoluble purple formazan crystals that can be solubilized and measured spectrophotometrically. |
| Intended Use | Quantitative measurement of cell viability and proliferation in mammalian cells following drug treatment, growth factor stimulation, or cytotoxic exposure in research and drug discovery applications. |
| Principle / Technology | Reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by NAD(P)H-dependent oxidoreductase enzymes in viable cells yields purple formazan crystals; dissolved crystals are measured by absorbance at 570 nm. |
| Detection Method | Colorimetric absorbance measurement at 570 nm using a microplate reader, with background subtraction at 630 nm |
| Sample Type | Adherent and suspension mammalian cell lines cultured in 96-well microplates |
| Performance Range / Specifications | Linear detection range: 500–100,000 cells per well (cell type-dependent); absorbance values typically 0.2–2.0 OD at 570 nm |
| Sensitivity / LOD | Approximately 500 viable cells per well for most mammalian lines |
| Specificity | MTT reduction activity is specific to metabolically active viable cells; dead or quiescent cells produce minimal signal; DMSO solvent control ensures formazan dissolution |
| Reaction Conditions / Protocol | Seed cells in 96-well plate and treat as required; add 10 μL MTT reagent (5 mg/mL) per 100 μL medium; incubate 2–4 hours at 37°C; remove medium and dissolve formazan with 100 μL DMSO or solubilization buffer; shake 10 minutes; read absorbance at 570 nm |
| Components / Formulation | MTT Reagent (lyophilized or solution at 5 mg/mL in PBS), Solubilization Solution (DMSO or acidified isopropanol with SDS), sterile PBS for reagent reconstitution, detailed protocol with troubleshooting guide |
| Storage Conditions | MTT reagent lyophilized: -20°C protected from light; MTT solution: 2–8°C protected from light, stable for 4 weeks; Solubilization Solution: room temperature |
| Shelf Life | 12 months from date of manufacture for lyophilized reagent |
| Package Specifications | 1,000 tests (96-well format), 5,000 tests, or 10,000 tests |
| Product Form | Lyophilized MTT with separate solubilization buffer |
| Quality Control | Each lot is tested for formazan yield using standard cell lines (HeLa, HEK293); linearity confirmed (R2 ≥0.98) across the specified cell range; endotoxin tested (<0.05 EU/test) |
| Key Features | Widely cited and cost-effective viability method; compatible with standard 96-well plate readers; no washing steps required before dye addition; signal stable for several hours after solubilization |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
