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| Product Name | Mouse Apolipoprotein E (Apo-E) ELISA Kit |
| Catalog No. | DDMD-QCY-0057 |
| Description | This kit is a competitive ELISA assay designed for quantitative detection of APOE levels in serum, plasma, tissue culture supernatants, and tissue homogenate samples. APOE (apolipoprotein E) is a lipid carrier crucial for cholesterol homeostasis. |
| Application | This kit can be widely applied in research fields such as the study of metabolic regulation mechanisms, the evaluation of atherosclerosis models, and animal experiments related to Alzheimer's disease. |
| Species | Mus musculus (Mouse) |
| Assay Type | Quantitative sandwich |
| Samples | Serum, plasma, cell culture supernates, tissue homogenates |
| Sample Volume | 50-100 uL |
| Detection Wavelength | 450 nm |
| Detection Range | 6.25-400 ng/mL |
| Sensitivity | 1.56 ng/mL |
| Test Time | 1-5 h |
| Composition of the Kit | A 96-well assay plate Two pieces of standard (freeze-dried) HRP-conjugated ApoE antibody (100 x concentrate) HRP-conjugate diluent (1 x 10 mL) Sample diluent (2 x 20 mL) Wash buffer (25x concentrate) (1 x 20 mL) TMB substrate (1x 10 mL) Stop solution (1 x 10 mL) Four adhesive strips An instruction manual |
| Self-provided Materials | Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set to 540 nm or 570 nm. Incubator providing stable culture conditions within a temperature range of 37°C ± 5°C. Centrifuge and vortex mixer. Absorbent paper for drying microplate wells. 50–300 μL multichannel pipette and pipette tips. Deionized or distilled water. Timer. Test tubes for dilution. |
| Storage | Store at 2-8°C. |
For research use only, not for clinical use.
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