| Product Name | Mouse Apolipoprotein B (APOB) ELISA Kit |
| Catalog No. | DDMD-QCY-0054 |
| Description | This kit is a competitive ELISA assay designed for quantitative detection of apolipoprotein B (APOB) in serum, plasma, and tissue homogenate samples. Apolipoprotein B (APOB) is a protein synthesized in the liver, primarily present in chylomicrons, low-density lipoproteins (LDL), and very low-density lipoproteins (VLDL), where it facilitates lipid transport. |
| Application | This kit can be used for studying lipid metabolism mechanisms in mouse models, conducting atherosclerosis-related experiments, or evaluating the efficacy of drug interventions. |
| Species | Mus musculus (Mouse) |
| Assay Type | Quantitative sandwich |
| Samples | Serum, plasma, tissue homogenates |
| Sample Volume | 50-100 uL |
| Detection Wavelength | 450 nm |
| Detection Range | 0.156-10 μg/mL |
| Sensitivity | 0.039 μg/mL |
| Test Time | 1-5 h |
| Composition of the Kit | A 96-well assay plate Two pieces of standard (freeze-dried) HRP-conjugated ApoB antibody (100 x concentrate) HRP-conjugate diluent (1 x 10 mL) Sample diluent (2 x 20 mL) Wash buffer (25x concentrate) TMB Substrate (1x 10 mL) Stop solution (1 x 10mL) Four adhesive strips (96 wells) An instruction manual |
| Self-provided Materials | Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set to 540 nm or 570 nm. Incubator providing stable culture conditions within a temperature range of 37°C ± 5°C. Centrifuge and vortex mixer. Absorbent paper for drying microplate wells. 50–300 μL multichannel pipette and pipette tips. Deionized or distilled water. Timer. Test tubes for dilution. |
| Storage | Store at 2-8°C. |
For research use only, not for clinical use.
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