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| Product Name | Modified Wright-Giemsa Stain for Peripheral Blood Smear |
| Catalog No. | ATRSA-0021 |
| Description | A modified Wright-Giemsa combined staining solution optimized for the differentiation of white blood cell types and assessment of red blood cell morphology in peripheral blood smears. The combination of Wright's stain and Giemsa stain in a single solution provides balanced staining of both nuclear detail and cytoplasmic granules, yielding clear morphological discrimination of neutrophils, eosinophils, basophils, lymphocytes, and monocytes in a single staining step. |
| Intended Use | Differential staining of peripheral blood films for white blood cell classification and red cell morphology evaluation. |
| Principle / Technology | Romanowsky-type polychromatic staining with azure B and eosin Y dyes |
| Detection Method | Brightfield light microscopy at 500× and 1000× (oil immersion) |
| Sample Type | Peripheral blood smears on glass slides, air-dried, methanol-fixed |
| Performance Range / Specifications | Staining time: 3–5 min for thin films; staining intensity adjustable by stain-to-buffer ratio |
| Sensitivity / LOD | Clear nuclear chromatin pattern and cytoplasmic granule color differentiation |
| Specificity | Eosinophilic granules stain orange-red; basophilic granules stain deep purple; neutrophilic granules stain lilac; lymphocyte cytoplasm pale blue |
| Reaction Conditions / Protocol | Fix blood smear in absolute methanol 1 min; stain with Wright-Giemsa working solution 3–5 min; add equal volume buffer pH 6.8, let stand 5–10 min; rinse with buffer; air dry |
| Components / Formulation | Modified Wright-Giemsa stain concentrate, phosphate buffer pH 6.8 (ready-to-use) |
| Storage Conditions | Stain: room temperature, protected from light; buffer: room temperature |
| Shelf Life | 24 months in sealed container |
| Package Specifications | 250 mL stain + 500 mL buffer |
| Product Form | Liquid stain concentrate |
| Quality Control | Each lot validated on normal donor peripheral blood smears for WBC differential staining quality |
| Key Features | Combined Wright-Giemsa formula eliminates the sequential two-step staining protocol of traditional methods |
| Purity | Dye content as specified; filtered to remove particulates |
| Concentration | As specified; ready-to-use or concentrate format |
| Activity / Unit Definition | Staining intensity and specificity validated on reference tissue/cell preparations |
| Molecular Weight | Varies by dye component as specified in product documentation |
| Source / Origin | Synthetic dyes and biological stains; certified by Biological Stain Commission where applicable |
| pH Range / Optimal pH | pH 6.0–7.0 for Romanowsky-type stains; varies by formulation |
| Shipping Conditions | Ambient temperature; protect from light and moisture |
| Expiration Date / Stability | 24 months in unopened container; protect from light |
| Regulatory / Compliance | For in vitro diagnostic use in histology and cytology; CE-marked options available |
| Compatibility | Compatible with formalin-fixed tissue sections, blood smears, and cytological preparations |
| Recommended Buffer System | Use with provided buffer or phosphate buffer pH 6.8 as specified |
| Application Notes / Precautions | Avoid contact with skin and clothing; stains are permanent; use appropriate PPE |
| Batch-to-Batch Consistency | Staining intensity and differential contrast validated on reference specimens per lot |
For research use only, not for clinical use.
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