Modified Wright-Giemsa Stain, Combined Single Solution
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Modified Wright-Giemsa Stain, Combined Single Solution

Cat.No: ATRSA-0014 Datasheet

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Product Name Modified Wright-Giemsa Stain, Combined Single Solution
Catalog No. ATRSA-0014
Description A combined Wright-Giemsa staining solution providing the advantages of both Romanowsky stains in a single reagent for the differentiation of hematopoietic cells in blood and bone marrow specimens.
Intended Use Staining peripheral blood and bone marrow smears for hematological evaluation of cell morphology, differential leukocyte count, and detection of hematopoietic abnormalities.
Principle / Technology Combines the rapid fixation and excellent cytoplasmic granule staining of Wright's stain with the enhanced nuclear detail and chromatin definition of Giemsa stain in a single methanolic solution.
Detection Method Light microscopy at 50x–100x oil immersion
Sample Type Peripheral blood smears, bone marrow aspirates, buffy coat preparations, cytospin slides
Performance Range / Specifications Five-part WBC differential and erythrocyte morphology assessment achievable with single staining procedure
Sensitivity / LOD N/A
Specificity Neutrophilic, eosinophilic, and basophilic granules distinctly stained; monocyte, lymphocyte, and blast nuclei clearly resolved
Reaction Conditions / Protocol Cover smear with stain for 30 sec to 1 min (fixation); add phosphate buffer pH 6.8 for 2–4 min; rinse with buffer; air dry.
Components / Formulation Modified Wright-Giemsa stain in methanol, phosphate buffer supplied separately
Storage Conditions 15–30°C, flammable, tightly sealed
Shelf Life 24 months
Package Specifications 500 mL, 1 L
Product Form Liquid, ready to use
Quality Control Staining validation with normal and abnormal blood smear reference slides, leukocyte differential accuracy
Key Features Combined Wright-Giemsa benefits in single reagent; rapid protocol; enhanced nuclear detail; ready to use; standard for hematology

For research use only, not for clinical use.

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