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| Product Name | Modified Masson Trichrome Staining Kit |
| Catalog No. | RERE-0250 |
| Description | Modified trichrome kit, also known as the ponceau acid fuchsin and aniline blue method, for staining collagen fibres. Nuclei are stained with a celestine blue and haematoxylin formulation, an improvement that sharpens the nuclear counterstain before the cytoplasmic dyes are applied. In the finished section collagen fibres appear blue, while muscle fibres, cytoplasm, erythrocytes, fibrin and keratin take the red dye, and nuclei read blue-brown. Store at 2-8 °C away from light; the reagents keep for 12 months. |
| Intended Use | Collagen staining with an improved nuclear counterstain for tissue sections |
| Principle / Technology | Celestine blue haematoxylin nuclear staining is followed by ponceau acid fuchsin and aniline blue differentiation |
| Specificity | 8 bottle set; celestine blue haematoxylin modified formulation; collagen fibres blue, muscle fibres, cytoplasm, erythrocytes, fibrin and keratin red, nuclei blue-brown; 12 month shelf life |
| Reaction Conditions / Protocol | Stain nuclei with the celestine blue haematoxylin reagent, then work through the acid fuchsin and aniline blue steps |
| Storage Conditions | 2-8 °C, protected from light |
| Package Specifications | 8 x 50 mL |
| Key Features | Modified nuclear stain; extended colour separation; 12 month shelf life |
For research use only, not for clinical use.
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