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| Product Name | Mitochondrion-Selective Red Fluorescent Probe, Fixation-Retaining (Ex/Em 581/644 nm) |
| Catalog No. | RERE-0011 |
| Description | This cell-permeant carbocyanine-based probe carries a weakly thiol-reactive chloromethyl group that anchors it inside active mitochondria. A simple incubation is enough for the dye to pass through the membrane and accumulate in functioning mitochondria, and once stained, the cells can be fixed with an aldehyde such as formaldehyde and permeabilized with a detergent such as Triton X-100 without losing the signal. Older membrane-potential dyes such as TMR and rhodamine 123 wash away once mitochondrial membrane potential collapses, which restricts them in experiments involving fixation or membrane-potential modifiers; this probe is built to avoid that limitation. Its red emission separates cleanly from green probes for double-staining work. |
| Intended Use | Selective labelling of active mitochondria in live cells, including workflows that continue through fixation and permeabilization, and double-staining with green probes. |
| Principle / Technology | The probe enters cells passively and concentrates in active mitochondria, where the chloromethyl group reacts weakly with thiols; this covalent anchoring retains the signal through fixation and permeabilization. |
| Detection Method | Red fluorescence at Ex 581 nm / Em 644 nm by fluorescence microscopy or imaging systems. |
| Sample Type | Live cultured cells with active mitochondria. |
| Performance Range / Specifications | Molecular formula C39H36Cl5N3; molecular weight 724 g/mol; appearance purple solid; Ex 581 nm / Em 644 nm; reactive group: weakly thiol-reactive chloromethyl; retained after formaldehyde fixation and Triton X-100 permeabilization. |
| Specificity | Targets active mitochondria; signal survives aldehyde fixation and detergent permeabilization. |
| Reaction Conditions / Protocol | Incubate live cells with the diluted probe, wash, then image directly or continue through fixation and permeabilization before imaging. |
| Components / Formulation | One vial of probe (50 μg). Solvents and buffers are supplied by the user. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 50 μg per vial |
| Product Form | Purple solid. |
| Key Features | - Signal survives formaldehyde fixation and Triton X-100 - Chloromethyl anchoring in active mitochondria - Red 581/644 nm emission separates from green probes - Overcomes wash-out limits of potential-sensitive dyes - Suited to double-staining designs |
| Activity / Unit Definition | Probe performance is demonstrated by mitochondrial labelling that persists through fixation. |
| Molecular Weight | Molecular formula C39H36Cl5N3; molecular weight 724 g/mol |
| Source / Origin | Synthetic small-molecule fluorescent probe, supplied as a solid. |
| Expiration Date / Stability | Stability is maintained by the recommended storage temperature together with continuous protection from light. |
| Regulatory / Compliance | Supplied for laboratory research purposes. |
| Compatibility | Read-outs run on routine fluorescence equipment without proprietary hardware. |
| Recommended Buffer System | Not included; prepare a DMSO or similar stock before staining. |
| Application Notes / Precautions | Protect from light at all times, both in storage and during labelling, and avoid repeated warming cycles. Prepare stock solutions fresh where possible and confirm the instrument filter set before starting an experiment. |
| Batch-to-Batch Consistency | Each production lot is released against fixed acceptance criteria, keeping performance consistent between orders. |
| Model Type | Organelle-selective fluorescent probe (mitochondria) |
| Starting Material / Tissue Site | Synthesised carbocyanine fluorophore with a chloromethyl reactive group |
| Delivery Standard | Pack size 50 μg |
| Turnaround Time | dispatch timing is settled at purchase. |
| Readouts / Downstream Assays | Red fluorescence at Ex 581/Em 644 nm marking active mitochondria. |
For research use only, not for clinical use.
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