- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Mitochondrion-Selective Orange Fluorescent Probe, Reduced Form (Ex/Em 554/576 nm) |
| Catalog No. | RERE-0013 |
| Description | This probe is the reduced (dihydro) derivative of the X-rosamine mitochondrial dye family, carrying the same weakly thiol-reactive chloromethyl group. Cells take it up by simple incubation, the dye accumulates in active mitochondria, and after aldehyde fixation or Triton X-100 permeabilization the signal stays put. As with the other members of this family, the orange emission separates well from green fluorophores for double staining, and unlike TMR or rhodamine 123 the signal is not lost when mitochondrial membrane potential collapses. |
| Intended Use | Selective labelling of active mitochondria in live cells, including workflows that continue through fixation and permeabilization, and double staining with green probes. |
| Principle / Technology | Passive entry into the cell followed by concentration in active mitochondria; the chloromethyl group reacts weakly with thiols and retains the dye through fixation. |
| Detection Method | Orange fluorescence at Ex 554 nm / Em 576 nm by fluorescence microscopy. |
| Sample Type | Live cultured cells with active mitochondria. |
| Performance Range / Specifications | CAS 167095-05-8; molecular formula C24H25ClN2O; molecular weight 392.93 g/mol; appearance pink solid; Ex 554 nm / Em 576 nm; reactive group: weakly thiol-reactive chloromethyl; reduced dihydro-X-rosamine derivative; fixation and permeabilization compatible. |
| Specificity | Targets active mitochondria; reduced form of the rosamine scaffold; signal survives fixation and permeabilization. |
| Reaction Conditions / Protocol | Incubate live cells with the diluted probe, wash, then image directly or continue through fixation and permeabilization before imaging. |
| Components / Formulation | One vial of probe (50 μg). Solvents and buffers are supplied by the user. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 50 μg per vial |
| Product Form | Pink solid. |
| Key Features | - Reduced dihydro-rosamine form of the mitochondrial family - Retained through fixation and permeabilization - Chloromethyl anchoring in active mitochondria - Orange 554/576 nm read-out - Effective with a simple one-step incubation |
| Activity / Unit Definition | Probe performance is demonstrated by mitochondrial labelling that persists through fixation. |
| Molecular Weight | CAS 167095-05-8; molecular formula C24H25ClN2O; molecular weight 392.93 g/mol. |
| Source / Origin | Synthetic small-molecule fluorescent probe, supplied as a solid. |
| pH Range / Optimal pH | Does not apply; pH is not a meaningful specification for this product type. |
| Expiration Date / Stability | Remains stable for the stated period provided the cold chain is not broken and light exposure is avoided. |
| Regulatory / Compliance | Restricted to research applications. |
| Compatibility | Works on common fluorescence platforms; match the filter set to the excitation/emission values listed for this product. |
| Recommended Buffer System | Not included; prepare a DMSO or similar stock before staining. |
| Application Notes / Precautions | Wear gloves when handling DMSO stocks, keep vials in the dark, and let chilled vials warm to room temperature before opening to avoid condensation. |
| Batch-to-Batch Consistency | Lot-to-lot consistency is controlled through defined manufacturing specifications; each lot ships with its release record. |
| Model Type | Organelle-selective fluorescent probe (mitochondria) |
| Starting Material / Tissue Site | Synthesised dihydro-X-rosamine fluorophore with a chloromethyl reactive group |
| Delivery Standard | Pack size 50 μg |
| Turnaround Time | No turnaround figure is published; shipping is arranged when the order is confirmed. |
| Readouts / Downstream Assays | Orange fluorescence at Ex 554/Em 576 nm marking active mitochondria. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
