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| Product Name | Mitochondrion-Selective Orange Fluorescent Probe, Oxidized Form (Ex/Em 554/576 nm) |
| Catalog No. | RERE-0012 |
| Description | An X-rosamine derivative in its oxidized form, carrying the weakly thiol-reactive chloromethyl group that marks mitochondria. Simple incubation lets the dye pass through the cell membrane and concentrate in active mitochondria, and once in place it survives aldehyde fixation and detergent permeabilization. The orange emission at 576 nm separates cleanly from green fluorophores, which suits double-staining designs; the probe's advantage over potential-sensitive dyes such as TMR and rhodamine 123 is that its signal does not wash out when membrane potential is lost. |
| Intended Use | Selective labelling of active mitochondria in live cells, including workflows that continue through fixation and permeabilization, and double staining with green probes. |
| Principle / Technology | Passive entry into the cell followed by concentration in active mitochondria; the chloromethyl group reacts weakly with thiols and holds the dye in place through fixation. |
| Detection Method | Orange fluorescence at Ex 554 nm / Em 576 nm by fluorescence microscopy. |
| Sample Type | Live cultured cells with active mitochondria. |
| Performance Range / Specifications | CAS 199116-50-2; molecular formula C24H24Cl2N2O; molecular weight 427.37 g/mol; appearance red solid; Ex 554 nm / Em 576 nm; reactive group: weakly thiol-reactive chloromethyl; oxidized X-rosamine derivative; fixation and permeabilization compatible. |
| Specificity | Targets active mitochondria; oxidized orange form; signal survives fixation and permeabilization. |
| Reaction Conditions / Protocol | Incubate live cells with the diluted probe, wash, then image directly or proceed through fixation and permeabilization before imaging. |
| Components / Formulation | One vial of probe (50 μg). Solvents and buffers are supplied by the user. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 50 μg per vial |
| Product Form | Red solid. |
| Key Features | - Oxidized orange form for 554/576 nm imaging - Retained through fixation and permeabilization - Chloromethyl anchoring in active mitochondria - Clean separation from green fluorophores - Effective with a simple one-step incubation |
| Activity / Unit Definition | Probe performance is demonstrated by mitochondrial labelling that persists through fixation. |
| Molecular Weight | CAS 199116-50-2; molecular formula C24H24Cl2N2O; molecular weight 427.37 g/mol. |
| Source / Origin | Synthetic small-molecule fluorescent probe, supplied as a solid. |
| Expiration Date / Stability | Stable through the stated shelf life when kept protected from light at the recommended storage temperature. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with standard fluorescence instrumentation - microscopes, plate readers, flow cytometers or imaging systems as appropriate to the format. |
| Recommended Buffer System | Not included; prepare a DMSO or similar stock before staining. |
| Application Notes / Precautions | Handle protected from light, minimise freeze-thaw cycles by preparing aliquots, and verify that the detection channel matches the dye before use. |
| Batch-to-Batch Consistency | Consistency between lots is maintained by fixed production controls and release testing against specification. |
| Model Type | Organelle-selective fluorescent probe (mitochondria) |
| Starting Material / Tissue Site | Synthesised X-rosamine fluorophore (oxidized form) with a chloromethyl reactive group |
| Delivery Standard | Pack size 50 μg |
| Readouts / Downstream Assays | Orange fluorescence at Ex 554/Em 576 nm marking active mitochondria. |
For research use only, not for clinical use.
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