| Product Name | Mitochondrial Membrane Potential and Apoptosis Detection Reagent |
| Catalog No. | CCAT-HMM-0028 |
| Description | This product is used in combination with the mitochondrial membrane potential-dependent red fluorescent probe Mito-Tracker Red CMXRos (also known as MitoTracker Red CMXRos) and the apoptosis green fluorescent probe Annexin V-FITC to detect mitochondrial membrane potential and apoptosis in cultured cells. Mito-Tracker Red CMXRos (mitochondrial red fluorescent probe) is a cell-permeable X-rosamine derivative (Chloromethyl-X-rosamine, abbreviated as CMXRos) that can specifically label mitochondria with biological activity within cells. In normal cells, Mito-Tracker Red CMXRos stains mitochondria with bright red fluorescence. However, during apoptosis or other processes, the mitochondrial membrane potential decreases, causing the red fluorescence of mitochondria to gradually weaken or even become red fluorescence-negative. |
| Application | This product simultaneously detects two important apoptosis detection indicators: changes in mitochondrial membrane potential and phosphatidylserine exposure. |
| Applicable Instruments | Flow cytometer, fluorescence microscope, or other fluorescence detection equipment |
| Materials to Bring | 1 × PBS, pH 7.2-7.6; sterile ddH₂O |
| Storage | Store at -20°C away from light. |
| Shelf Life | 1 year |
| Notes | Although testing has shown that repeated freeze-thaw cycles (5 times) do not significantly affect the detection performance of Annexin V-FITC, it is recommended to store the product at 4°C within 3-6 months to achieve optimal results, and to avoid repeated freeze-thaw cycles whenever possible. If cells are contaminated with bacteria or fungi, this can severely impair detection performance. It is advisable to perform the assay promptly after staining, as prolonged incubation may lead to an increase in the number of apoptotic or necrotic cells. If trypsin was used during cell collection, care should be taken to remove any residual trypsin. Residual trypsin may degrade Annexin V-FITC, leading to staining failure. Fluorescent substances are prone to quenching. When performing fluorescence observations, minimize observation time and ensure light-protected storage during handling and storage. When using Annexin V-FITC for flow cytometry analysis, if the signal is excessively strong and cannot be improved by adjusting settings or parameters, dilute Annexin V-FITC with PBS at a ratio of 3–10 times before testing. For your safety and health, please wear a lab coat and disposable gloves during handling. |
For research use only, not for clinical use.
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