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| Product Name | Mitochondria Red Fluorescent Probe (CMXRos) |
| Catalog No. | RESK-0547 |
| Description | Mitochondria Red Fluorescent Probe (CMXRos) is a cell-permeable X-rosamine derivative bearing a weakly thiol-reactive chloromethyl group for mitochondrial labeling. The product is an oxidized red fluorescent dye (Ex = 579 nm, Em = 599 nm). After simply incubating cells with the probe, it passively diffuses across the cell membrane and directly accumulates in active mitochondria. Once mitochondria are stained, cells can be fixed with aldehyde fixatives such as formaldehyde and permeabilized with detergents such as Triton X-100 according to downstream experimental needs, and the probe is retained in the cells. Its red fluorescence is well resolved from green fluorescent probes, making the product well suited for dual-labeling experiments. Unlike conventional mitochondrial fluorescent probes such as TMR and rhodamine 123, which readily accumulate in functional mitochondria but are washed out once the mitochondrial membrane potential is lost, this probe is retained after staining, extending its utility to experiments that require aldehyde fixation or involve factors affecting the mitochondrial energy state. |
| Intended Use | Selective red fluorescent staining of active mitochondria in live cells, including experiments requiring subsequent aldehyde fixation and permeabilization; suitable for flow cytometry and dual labeling with green fluorescent probes. |
| Principle / Technology | The cell-permeable oxidized X-rosamine dye passively crosses the cell membrane and accumulates directly in active mitochondria. Its weakly thiol-reactive chloromethyl group promotes covalent retention in mitochondria, so the signal is preserved after aldehyde fixation and permeabilization. |
| Detection Method | Fluorescence microscopy, confocal microscopy, microplate reader, or flow cytometry (PE or FL2 channel); Ex/Em = 579/599 nm. |
| Sample Type | Live cultured cells. |
| Performance Range / Specifications | CAS No.: 167095-09-2 Molecular formula: C32H32Cl2N2O Molecular weight: 531.52 Ex: 579 nm Em: 599 nm Appearance: purple solid |
| Specificity | Selective for mitochondria; the dye directly accumulates in active mitochondria of live cells. |
| Reaction Conditions / Protocol | Simply incubate cells with the probe; the dye passively crosses the cell membrane and directly accumulates in active mitochondria. After staining, cells may be fixed with aldehyde fixatives (e.g., formaldehyde) and permeabilized (e.g., Triton X-100) as required. |
| Components / Formulation | Single vial of Mitochondria Red Fluorescent Probe (CMXRos) solid. |
| Storage Conditions | Store at -20 °C, protected from light and kept dry. |
| Shelf Life | 1 year when stored as recommended. |
| Package Specifications | 50 μg |
| Product Form | Purple solid. |
| Molecular Weight | 531.52 |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for 1 year when stored at -20 °C, protected from light and kept dry. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for flow cytometry (detect in the PE or FL2 channel); compatible with aldehyde fixation (e.g., formaldehyde) and detergent permeabilization (e.g., Triton X-100) after staining; suitable for dual labeling with green fluorescent probes. |
| Application Notes / Precautions | For flow cytometric detection, use the PE or FL2 channel. Unlike conventional probes such as TMR and rhodamine 123, the stained probe is retained after aldehyde fixation and is not washed out upon loss of mitochondrial membrane potential. |
For research use only, not for clinical use.
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