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| Product Name | Mitochondria Red Fluorescent Probe (CM-H2XRos) |
| Catalog No. | RESK-0546 |
| Description | Mitochondria Red Fluorescent Probe (CM-H2XRos) is a cell-permeable dihydro-X-rosamine derivative bearing a weakly thiol-reactive chloromethyl group for mitochondrial labeling. The probe is a reduced, essentially non-fluorescent red dye (Ex = 579 nm, Em = 599 nm). After simply incubating cells with the probe, it passively diffuses across the cell membrane and is oxidized inside the cell to a fluorescent, mitochondria-selective probe that accumulates in active mitochondria. Once mitochondria are stained, cells can be fixed with aldehyde fixatives such as formaldehyde and permeabilized with detergents such as Triton X-100 according to downstream experimental needs, and the probe is retained in the cells. Its red fluorescence is well resolved from green fluorescent probes, making the product well suited for dual-labeling experiments. Unlike conventional mitochondrial fluorescent probes such as TMR and rhodamine 123, which readily accumulate in functional mitochondria but are washed out once the mitochondrial membrane potential is lost, this probe is retained after staining, extending its utility to experiments that require aldehyde fixation or involve factors affecting the mitochondrial energy state. |
| Intended Use | Selective fluorescent staining of mitochondria in live cells, including experiments that require subsequent aldehyde fixation and permeabilization; suitable for dual labeling with green fluorescent probes. |
| Principle / Technology | The cell-permeable reduced dye is non-fluorescent until it is oxidized inside cells to a fluorescent, mitochondria-selective form. It accumulates in active mitochondria in a membrane-potential-dependent manner, and its weakly thiol-reactive chloromethyl group helps retain the probe in mitochondria after fixation. |
| Detection Method | Fluorescence microscopy, confocal microscopy, or flow cytometry (recommended); detection on a fluorescence microplate reader is also possible (Ex/Em = 579/599 nm). |
| Sample Type | Live cultured cells. |
| Performance Range / Specifications | CAS No.: 167095-08-1 Molecular formula: C32H33ClN2O Molecular weight: 497.08 Ex/Em: 579/599 nm Appearance: purple solid |
| Specificity | Selective for mitochondria; intracellular oxidation generates a fluorescent, mitochondria-selective probe that accumulates in active mitochondria. |
| Reaction Conditions / Protocol | Simply incubate cells with the probe; the dye passively crosses the cell membrane and is oxidized intracellularly to a fluorescent product that accumulates in active mitochondria. After staining, cells may be fixed with aldehyde fixatives (e.g., formaldehyde) and permeabilized (e.g., Triton X-100) as required. |
| Components / Formulation | Single vial of Mitochondria Red Fluorescent Probe (CM-H2XRos) solid. |
| Storage Conditions | Store at -20 °C, protected from light and kept dry. |
| Shelf Life | 1 year when stored as recommended. |
| Package Specifications | 50 μg |
| Product Form | Purple solid. |
| Molecular Weight | 497.08 |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for 1 year when stored at -20 °C, protected from light and kept dry. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with aldehyde fixation (e.g., formaldehyde) and detergent permeabilization (e.g., Triton X-100) after staining; suitable for dual labeling with green fluorescent probes. |
| Application Notes / Precautions | The probe itself is essentially non-fluorescent and fluoresces only after intracellular oxidation. Staining can be read on a microplate reader, but fluorescence microscopy, confocal microscopy, or flow cytometry is recommended because plate readers are generally less sensitive; carefully optimize labeling time and concentration. Mitochondrial accumulation of the oxidized dye depends on membrane potential. Unlike conventional probes such as TMR and rhodamine 123, the stained probe is retained after aldehyde fixation. |
For research use only, not for clinical use.
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