- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Mitochondria Green Fluorescent Probe (FM) |
| Catalog No. | RESK-0548 |
| Description | Mitochondria Green Fluorescent Probe (FM) is a cell-permeable carbocyanine-structure derivative bearing a weakly thiol-reactive chloromethyl group for mitochondrial labeling. It is a bright green fluorescent probe (Ex = 490 nm, Em = 523 nm) that is virtually non-fluorescent in aqueous solution and fluoresces only upon accumulating in the lipid environment of mitochondria, resulting in almost negligible background fluorescence. The workflow is extremely simple: just incubate cells with the probe, which passively crosses the cell membrane and directly accumulates in active mitochondria; staining can be observed directly without a washing step. Unlike mitochondria-selective red rosamine probes, the ability of this probe to localize to mitochondria is not affected by the mitochondrial membrane potential, making it a potential tool for quantifying mitochondrial mass. The dye is not stable after aldehyde fixation, so it is more suitable for live-cell staining. Unlike conventional mitochondrial fluorescent probes such as TMR and rhodamine 123, which readily accumulate in functional mitochondria but are washed out once the mitochondrial membrane potential is lost, this probe retains mitochondrial localization regardless of membrane potential. |
| Intended Use | Green fluorescent staining of mitochondria in live cells; because localization is independent of mitochondrial membrane potential, the probe can serve as a tool for quantifying mitochondrial mass. |
| Principle / Technology | The cell-permeable carbocyanine-based probe is virtually non-fluorescent in aqueous solution and becomes brightly fluorescent only in the lipid environment of mitochondria. Mitochondrial localization is independent of membrane potential, and the weakly thiol-reactive chloromethyl group supports mitochondrial labeling. |
| Detection Method | Fluorescence microscopy, confocal microscopy, flow cytometry, or fluorescence microplate reader; Ex/Em = 490/523 nm. |
| Sample Type | Live cultured cells. |
| Performance Range / Specifications | CAS No.: 201860-17-5 Molecular formula: C34H28Cl5N3O Molecular weight: 671.88 Ex: 490 nm Em: 523 nm Appearance: red solid Recommended working concentration: approximately 50-200 nM |
| Specificity | Selective for mitochondria; fluorescence is generated only upon accumulation in the mitochondrial lipid environment, with almost negligible background. Mitochondrial localization is independent of membrane potential. |
| Reaction Conditions / Protocol | Incubate live cells with the probe; it passively crosses the cell membrane and directly accumulates in mitochondria. No washing step is required before observation. Recommended working concentration is approximately 50-200 nM. |
| Components / Formulation | Single vial of Mitochondria Green Fluorescent Probe (FM) solid. |
| Storage Conditions | Store at -20 °C, protected from light and kept dry. |
| Shelf Life | 2 years when stored as recommended. |
| Package Specifications | 50 μg |
| Product Form | Red solid. |
| Concentration | Recommended working concentration: approximately 50-200 nM. |
| Molecular Weight | 671.88 |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for 2 years when stored at -20 °C, protected from light and kept dry. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable only for live-cell staining; not compatible with fixation before or after staining, because fixation of stained live cells leads to loss of the fluorescent signal. |
| Recommended Buffer System | If HBSS is unavailable, PBS or serum-free medium can be used as a substitute; for flow cytometry, add a sufficient volume (e.g., 100-200 uL). |
| Application Notes / Precautions | Staining of structures other than mitochondria usually indicates that the probe concentration is too high; most organic dyes should be used at low concentrations. The recommended working concentration of this probe is very low, approximately 50-200 nM; higher concentrations can cause background fluorescence and non-mitochondrial staining. The dye is not stable after aldehyde fixation and is therefore recommended for live-cell staining only; it is not compatible with staining after fixation or fixation after staining. Staining results can be read on a microplate reader, but fluorescence microscopy, confocal microscopy, or flow cytometry is recommended where possible, because plate readers are generally less sensitive; carefully optimize labeling time and concentration. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
