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| Product Name | Microcentrifuge Tubes, 1.5 mL, RNase/DNase-Free |
| Catalog No. | IGM-HMM-0048 |
| Description | Crystal-clear polypropylene microcentrifuge tubes with attached snap-cap and frosted writing surface. Certified free of detectable RNase, DNase, and human genomic DNA. Withstands centrifugation up to 25,000 x g in standard microcentrifuge rotors. |
| Intended Use | Routine molecular biology sample handling including nucleic acid storage, enzyme reaction preparation, PCR master mix aliquoting, protein extraction, and general laboratory sample containment at microliter volumes. |
| Principle / Technology | Conical bottom design ensures complete sample recovery and efficient pelleting. Positive-seal attached cap with audible click closure prevents sample loss during boiling, incubation, and centrifugation. Frosted lid and side surface for permanent marking. |
| Detection Method | Centrifugation integrity at 25,000 x g; nuclease activity by fluorogenic substrate assay; seal integrity by dye leak test at 95 C |
| Sample Type | DNA, RNA, protein solutions, cell lysates, enzyme reactions, PCR products, reagent aliquots, bacterial pellets |
| Performance Range / Specifications | Working volume 0.1-1.5 mL; maximum RCF 25,000 x g; seal integrity to 95 C for 10 minutes; graduation markings at 0.1, 0.5, 1.0, 1.5 mL |
| Sensitivity / LOD | RNase detection limit <0.01 ng/mL; DNase <0.05 pg/uL; human DNA <0.1 pg/uL by qPCR |
| Specificity | No PCR inhibition detected; no heavy metal contamination; extractable organics below detection limit |
| Reaction Conditions / Protocol | Open cap by thumb pressure on cap edge; pipette sample; close cap firmly until audible click confirms sealing. |
| Components / Formulation | USP Class VI virgin polypropylene, no additives or slip agents; non-pyrogenic; silicone-free manufacturing |
| Storage Conditions | 15-30 C, dry environment; tubes may be autoclaved before use |
| Shelf Life | 60 months from manufacture date |
| Package Specifications | 500 tubes per bag, 10 bags per case; 250 tubes per tube rack box, 10 boxes per case; 1000 tubes bulk bag |
| Product Form | 1.5 mL conical microcentrifuge tube, natural clear, with attached snap-cap, bulk or racked |
| Quality Control | RNase/DNase certification per lot; centrifugation integrity tested; cap seal leak test; dimensional inspection; bioburden monitoring |
| Key Features | Crystal clear for easy sample visualization; RNase/DNase-free certified; autoclavable; graduated markings; frosted writing area; secure snap-cap with positive closure |
| Purity | Certified RNase-free, DNase-free, human DNA-free, PCR inhibitor-free, pyrogen-free; nuclease testing per production lot |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Medical-grade polypropylene resin; injection molded in ISO Class 8 cleanroom per ISO 13485 |
| pH Range / Optimal pH | Chemically resistant pH 1-14; withstands phenol, chloroform, ethanol, isopropanol, DMSO, and common laboratory solvents |
| Shipping Conditions | Ambient; avoid crushing of tube bags during transit |
| Expiration Date / Stability | 5 years from manufacture; polymer stability validated throughout shelf life |
| Regulatory / Compliance | ISO 13485:2016; CE marked; compliant with USP <661>; FDA 21 CFR 177.1520 for food contact polymer |
| Compatibility | Fits standard microcentrifuge rotors for 1.5/2.0 mL tubes (Eppendorf, Sorvall, Beckman, and generic); compatible with thermomixers, heating blocks, and floating tube racks |
| Recommended Buffer System | Compatible with all standard molecular biology buffers; resistant to denaturing solutions (guanidine thiocyanate, urea, formamide) |
| Application Notes / Precautions | For RNA work, use freshly opened tube directly from sealed packaging; do not touch inner surface; pre-chill tubes on ice for heat-sensitive samples; verify cap is fully closed before vortex mixing or centrifugation |
| Batch-to-Batch Consistency | Tube weight +/- 0.02 g; wall thickness uniform within +/- 0.03 mm; centrifugation integrity tested with water-filled tubes in 30-tube rotor per batch sample |
For research use only, not for clinical use.
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