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| Product Name | Methylene Blue Staining Solution, 0.5% in 1% Borax |
| Catalog No. | ATRSA-0015 |
| Description | A polychrome methylene blue solution for bacteriological staining, demonstrating metachromatic granules in Corynebacterium diphtheriae (Albert's stain), and for reticulocyte enumeration in blood specimens. |
| Intended Use | Microscopic detection of metachromatic granules (volutin) in Corynebacterium species; supravital staining of reticulocytes in peripheral blood; general bacterial morphology staining in microbiology. |
| Principle / Technology | Methylene blue in alkaline borax solution forms polychrome oxidation products (azure dyes) that produce metachromatic staining of volutin granules (purple-red) against a blue bacterial cytoplasm. |
| Detection Method | Bright-field light microscopy at 100x oil immersion |
| Sample Type | Bacterial smears from culture plates, peripheral blood for reticulocyte count, throat swabs for Albert's stain |
| Performance Range / Specifications | Metachromatic granules distinctly visible in C. diphtheriae; reticulocytes stained with granular blue precipitate |
| Sensitivity / LOD | N/A |
| Specificity | Metachromatic: volutin granules in Corynebacterium spp.; orthochromatic: bacterial cytoplasm, nuclei, reticulocyte RNA |
| Reaction Conditions / Protocol | Fix bacterial smear by heat; apply stain for 1–3 min; rinse with water; blot dry. For reticulocytes: mix equal volumes of blood and stain; incubate 15 min; prepare smear. |
| Components / Formulation | Methylene Blue chloride 0.5% w/v, Borax (sodium tetraborate) 1% w/v, in deionized water |
| Storage Conditions | 15–30°C, protect from light |
| Shelf Life | 24 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, ready to use |
| Quality Control | Metachromatic staining validation with C. diphtheriae reference strain, reticulocyte staining with normal blood |
| Key Features | Ready to use; polychrome properties from borax aging; dual application (bacteriology and hematology); economical |
For research use only, not for clinical use.
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