Masson Trichrome Staining Kit, Fast Green Method
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Masson Trichrome Staining Kit, Fast Green Method

Cat.No: RERE-0284 Datasheet

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Product Name Masson Trichrome Staining Kit, Fast Green Method
Catalog No. RERE-0284
Description Seven-bottle Masson trichrome kit that uses the fast green variant of the classical protocol, including a Weigert haematoxylin step for nuclear staining. Collagen fibers appear blue, nuclei blue brown, and muscle fibers along with red blood cells take red tones, so fibrotic remodeling can be judged against cellular detail on the same slide. Nuclei are easier to differentiate after the haematoxylin step, which improves the separation of collagen from muscle. Shelf life is 12 months at room temperature with light protection.
Intended Use Collagen fiber staining and fibrosis assessment in tissue sections
Principle / Technology Weigert haematoxylin stains nuclei first; anionic dyes then differentiate collagen in blue from muscle and cytoplasmic proteins in red
Specificity Fast green method with Weigert haematoxylin; collagen blue, nuclei blue brown, muscle fibers and red blood cells red; 12 month shelf life
Reaction Conditions / Protocol Run the seven reagent steps in order, from haematoxylin through the dye mixtures to differentiation, then dehydrate and mount
Storage Conditions Room temperature, protected from light
Package Specifications 7 × 50 mL / 7 × 100 mL
Key Features Fast green variant of the classical protocol; clear collagen versus muscle separation; 12 month shelf life

For research use only, not for clinical use.

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