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| Product Name | Magnetic Bead-Based Viral DNA/RNA Extraction Kit |
| Catalog No. | DREK-0001 |
| Description | A magnetic silica bead-based nucleic acid extraction kit optimized for the simultaneous recovery of viral DNA and RNA from cell-free biological fluids. The combination of chaotropic lysis chemistry and optimized binding buffer composition ensures high-efficiency capture of both genomic and fragmented viral nucleic acids. Purified nucleic acids are suitable for downstream real-time PCR, digital PCR, and next-generation sequencing without further purification. |
| Intended Use | Simultaneous extraction of viral DNA and RNA from plasma, serum, cerebrospinal fluid, bronchoalveolar lavage fluid, and other cell-free clinical specimens for molecular diagnostic testing of viral pathogens including SARS-CoV-2, influenza, hepatitis viruses, cytomegalovirus, and Epstein-Barr virus. |
| Principle / Technology | Viral particles are lysed in a guanidine isothiocyanate-based buffer containing carrier RNA to enhance binding and protect low-abundance targets. Nucleic acids are released and selectively bound to silica-coated magnetic beads under high-chaotrope conditions. After magnetic separation and ethanol-based washes, purified viral DNA and RNA are eluted in nuclease-free water or low-salt buffer under mild heating. |
| Detection Method | UV spectrophotometry at 260/280 nm for concentration and purity; fluorometric quantitation with Qubit or equivalent dsDNA/RNA-specific dyes; Bioanalyzer or TapeStation for fragment size analysis; qPCR or RT-qPCR with pathogen-specific primer-probe sets for functional recovery validation |
| Sample Type | Human plasma (EDTA, citrate, or heparin anticoagulated), serum, cerebrospinal fluid, bronchoalveolar lavage fluid, nasopharyngeal swab transport medium, saliva, urine supernatant, and cell culture supernatant |
| Performance Range / Specifications | Input volume: 200-1000 µL; typical recovery from 200 µL plasma: ≥80% for viral targets ≥200 copies/mL; A260/A280: 1.7-2.0; elution volume: 30-60 µL; carrier RNA included to protect low-abundance nucleic acids; internal control compatibility for extraction control monitoring |
| Sensitivity / LOD | Limit of detection: 50-100 copies/mL for DNA viruses and 100-200 copies/mL for RNA viruses from 200 µL plasma input; internal control consistently detected down to 10 copies per reaction |
| Specificity | Simultaneously recovers both DNA and RNA viral genomes without DNase or RNase treatment; minimal host genomic DNA carryover; carrier RNA supplementation ensures reliable recovery of picogram-level viral nucleic acids; no detectable cross-contamination between samples verified by negative control interspersion |
| Reaction Conditions / Protocol | Lyse 200-1000 µL sample with lysis/binding buffer and carrier RNA at room temperature for 10 minutes; add proteinase K if required for specific sample types; add magnetic bead suspension and isopropanol; incubate 5-10 minutes with intermittent mixing; separate beads on magnetic rack; wash twice with wash buffer 1 and twice with wash buffer 2; air-dry beads briefly; elute in 30-60 µL nuclease-free water at 56°C for 5 minutes; total processing time: 35-60 minutes for 1-24 samples |
| Components / Formulation | Lysis/binding buffer (guanidine isothiocyanate, Tris-HCl, EDTA, Triton X-100), carrier RNA (poly-A RNA lyophilized), proteinase K solution, magnetic silica bead suspension (50 mg/mL), wash buffer 1 concentrate (guanidine hydrochloride, Tris-HCl, NaCl, ethanol), wash buffer 2 concentrate (Tris-HCl, NaCl, ethanol), elution buffer (10 mM Tris-HCl pH 8.0, 0.1 mM EDTA), nuclease-free water, magnetic separation rack |
| Storage Conditions | Magnetic beads and buffers at room temperature (15-25°C); proteinase K at 2-8°C after reconstitution; carrier RNA at -20°C after reconstitution; protect lysis/binding buffer from direct sunlight; avoid repeated freeze-thaw cycles of carrier RNA |
| Shelf Life | 18 months from date of manufacture for buffers and magnetic beads; reconstituted proteinase K stable for 12 months at 2-8°C; reconstituted carrier RNA stable for 6 months at -20°C |
| Package Specifications | 50 preparations and 200 preparations; includes magnetic beads, buffers, carrier RNA, and nuclease-free water; magnetic separation rack available separately |
| Product Form | Liquid buffers; magnetic bead suspension; lyophilized carrier RNA and proteinase K |
| Quality Control | Each lot validated with quantified viral reference materials: recovery ≥80% for HCV RNA and HBV DNA at 10^3 IU/mL; A260/A280: 1.7-2.0; absence of DNase and RNase confirmed; qPCR inhibition tested with serial dilutions; endotoxin <0.05 EU per elution; lot release includes inter-sample cross-contamination testing |
| Key Features | Simultaneous DNA and RNA recovery without splitting sample; optimized for low-titer viral targets with carrier RNA supplementation; compatible with manual processing and open-deck liquid handling automation; 30-minute protocol from sample to eluate; internal control compatibility built into buffer system; post-elution magnetic bead removal ensures no bead carryover |
| Purity | A260/A280: 1.7-2.0; A260/A230: >1.8 |
| Concentration | Elution volume 30-60 µL; typical nucleic acid concentration 5-30 ng/µL from plasma samples with moderate viral load |
| Activity / Unit Definition | Not applicable for extraction kits |
| Molecular Weight | Not applicable |
| Source / Origin | Magnetic silica beads are synthetic; recombinantly expressed proteinase K from Tritirachium album expressed in Pichia pastoris; carrier RNA is synthetic poly-A RNA |
| pH Range / Optimal pH | Lysis/binding buffer pH 6.0-6.5; wash buffer 1 pH 6.5-7.0; wash buffer 2 pH 7.0-7.5; elution buffer pH 8.0-8.5 |
| Shipping Conditions | Ambient temperature shipping for dry components; cold packs recommended for proteinase K during extended transit in hot climates |
| Expiration Date / Stability | 18 months unopened; performance verified at 0, 6, 12, 18 months by accelerated stability testing at 37°C equivalent; after first use, buffers stable for 12 months when stored properly |
| Regulatory / Compliance | For laboratory research use only; not intended for diagnostic procedures; manufactured under ISO 13485 quality management principles |
| Compatibility | Compatible with KingFisher Flex, KingFisher Duo, KingFisher Presto, and other open magnetic rod-based automated extraction platforms; eluates suitable for all major qPCR, RT-qPCR, digital PCR, and NGS library preparation systems |
| Recommended Buffer System | Guanidine isothiocyanate-based lysis; Tris-HCl buffered wash and elution solutions; carrier RNA supplementation in lysis buffer |
| Application Notes / Precautions | For heparinized plasma samples, pretreatment with heparinase may improve qPCR performance; avoid over-drying magnetic beads after final wash to prevent reduced elution efficiency; vortex magnetic beads thoroughly before each use to ensure homogeneous suspension; use low-retention tubes for eluate storage to minimize nucleic acid binding to tube walls |
| Batch-to-Batch Consistency | Within-lot CV for viral target recovery <10%; between-lot CV <15%; magnetic bead binding capacity verified per lot by salmon sperm DNA titration; pH and conductivity checked for every buffer batch |
For research use only, not for clinical use.
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