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| Product Name | Lysosome-Selective Yellow Fluorescent Probe (Ex/Em 465/535 nm) |
| Catalog No. | RERE-0016 |
| Description | A yellow-emitting member of the acidotropic lysosome tracer family. The weak-base fluorophore enters live cells freely and accumulates in acidic spherical organelles, with labelling effective at nanomolar concentrations. Working at these low levels helps keep the cells undisturbed, and the family's colour options let the probe be combined with other labels in multi-colour experiments. The page lists observation of lysosomal biosynthesis and related disease mechanisms as the main applications. |
| Intended Use | Selective labelling of acidic organelles in live cells for lysosome biology, biosynthesis tracking and related pathology studies. |
| Principle / Technology | The fluorophore-linked weak base permeates the cell membrane and accumulates in acidic organelles through protonation and membrane retention. |
| Detection Method | Yellow fluorescence at Ex 465 nm / Em 535 nm by fluorescence microscopy. |
| Sample Type | Live cultured cells containing lysosomes. |
| Performance Range / Specifications | CAS 1064123-31-4; molecular formula C16H24N4O4; molecular weight 364.4 g/mol; appearance orange solution; Ex/Em 465/535 nm; effective at nanomolar concentrations; supplied as 1 mM stock in anhydrous DMSO. |
| Sensitivity / LOD | Effective labelling at nanomolar working levels; no numeric detection limit is quoted. |
| Specificity | Selective for acidic organelles; yellow emission placed between green and red channels for panel flexibility. |
| Reaction Conditions / Protocol | Dilute the 1 mM DMSO stock to a nanomolar working solution, incubate live cells, wash gently if required, then image on the yellow channel. |
| Components / Formulation | One vial of 50 μL probe solution (1 mM in anhydrous DMSO). |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 50 μL per vial |
| Product Form | Orange solution in anhydrous DMSO. |
| Key Features | - Yellow 465/535 nm read-out between green and red channels - Selective acidic-organelle labelling in live cells - Effective at nanomolar working concentrations - Suits multi-colour organelle panels - Supplied as a 1 mM anhydrous DMSO stock |
| Concentration | 1 mM stock solution in anhydrous DMSO. |
| Activity / Unit Definition | Probe performance is demonstrated by selective nanomolar labelling of lysosomes. |
| Molecular Weight | CAS 1064123-31-4; molecular formula C16H24N4O4; molecular weight 364.4 g/mol. |
| Source / Origin | Synthetic small-molecule fluorescent probe, supplied as a DMSO stock. |
| pH Range / Optimal pH | Does not apply; pH is not a meaningful specification for this product type. |
| Expiration Date / Stability | Remains stable for the stated period provided the cold chain is not broken and light exposure is avoided. |
| Regulatory / Compliance | Restricted to research applications. |
| Compatibility | Works on common fluorescence platforms; match the filter set to the excitation/emission values listed for this product. |
| Recommended Buffer System | Supplied in anhydrous DMSO as a 1 mM stock; working solutions are prepared by the user. |
| Application Notes / Precautions | Wear gloves when handling DMSO stocks, keep vials in the dark, and let chilled vials warm to room temperature before opening to avoid condensation. |
| Batch-to-Batch Consistency | Lot-to-lot consistency is controlled through defined manufacturing specifications; each lot ships with its release record. |
| Model Type | Organelle-selective fluorescent probe (lysosome) |
| Starting Material / Tissue Site | Synthesised acidotropic weak-base fluorophore |
| Delivery Standard | 50 μL vial at 1 mM |
| Turnaround Time | No turnaround figure is published; shipping is arranged when the order is confirmed. |
| Readouts / Downstream Assays | Yellow fluorescence at Ex 465/Em 535 nm marking acidic organelles. |
For research use only, not for clinical use.
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