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| Product Name | Lysosome-Selective Deep Red Fluorescent Probe (Ex/Em 647/668 nm) |
| Catalog No. | RERE-0017 |
| Description | This deep-red probe stains acidic compartments of live cells in the far-red window. Its excitation and emission at 647/668 nm match the Cy5 filter set present on most fluorescence instruments, and the long wavelength keeps well clear of cellular autofluorescence and of green and orange labels, which suits multi-colour experiments. Like its family members it is a fluorophore-linked weak base that permeates the membrane and accumulates in acidic organelles, at nanomolar working levels, and it is supplied as a 1 mM stock in anhydrous DMSO. |
| Intended Use | Selective labelling of acidic organelles in live cells in the far-red window, suited to multi-colour panels and low-autofluorescence imaging. |
| Principle / Technology | The fluorophore-linked weak base permeates the cell membrane and accumulates in acidic organelles through protonation and membrane retention. |
| Detection Method | Deep red fluorescence at Ex 647 nm / Em 668 nm, read on the Cy5 filter set. |
| Sample Type | Live cultured cells containing lysosomes. |
| Performance Range / Specifications | Ex/Em 647/668 nm; Cy5 filter compatible; supplied at 1 mM in anhydrous DMSO |
| Sensitivity / LOD | Effective labelling at nanomolar working levels; no numeric detection limit is quoted. |
| Specificity | Selective for acidic organelles; far-red emission minimising autofluorescence and overlap with shorter-wavelength labels. |
| Reaction Conditions / Protocol | Dilute the 1 mM DMSO stock to a nanomolar working solution, incubate live cells, wash gently if required, then image on the Cy5 channel. |
| Components / Formulation | One vial of 50 μL probe solution (1 mM in anhydrous DMSO). |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 50 μL per vial |
| Product Form | Solution in anhydrous DMSO (deep red appearance). |
| Key Features | - Far-red 647/668 nm read-out on the Cy5 filter set - Low cellular autofluorescence in the deep-red window - Selective acidic-organelle labelling in live cells - Pairs with green and orange labels without overlap - Supplied as a 1 mM anhydrous DMSO stock |
| Concentration | 1 mM stock solution in anhydrous DMSO. |
| Activity / Unit Definition | Probe performance is demonstrated by selective nanomolar labelling of lysosomes. |
| Source / Origin | Synthetic small-molecule fluorescent probe, supplied as a DMSO stock. |
| Expiration Date / Stability | Stability is maintained by the recommended storage temperature together with continuous protection from light. |
| Regulatory / Compliance | Supplied for laboratory research purposes. |
| Compatibility | Read-outs run on routine fluorescence equipment without proprietary hardware. |
| Recommended Buffer System | Supplied in anhydrous DMSO as a 1 mM stock; working solutions are prepared by the user. |
| Application Notes / Precautions | Protect from light at all times, both in storage and during labelling, and avoid repeated warming cycles. Prepare stock solutions fresh where possible and confirm the instrument filter set before starting an experiment. |
| Batch-to-Batch Consistency | Each production lot is released against fixed acceptance criteria, keeping performance consistent between orders. |
| Model Type | Organelle-selective fluorescent probe (lysosome) |
| Starting Material / Tissue Site | Synthesised far-red acidotropic weak-base fluorophore |
| Delivery Standard | 50 μL vial at 1 mM |
| Turnaround Time | dispatch timing is settled at purchase. |
| Readouts / Downstream Assays | Deep red fluorescence at Ex 647/Em 668 nm marking acidic organelles. |
For research use only, not for clinical use.
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