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| Product Name | Lysosome-Selective Blue Fluorescent Probe (Ex/Em 373/422 nm) |
| Catalog No. | RERE-0015 |
| Description | This blue probe stains acidic compartments selectively in living cells. A weak base tethered to a fluorophore, it crosses the membrane freely and settles in spherical acidic organelles, most probably by full protonation and retention on the organelle membrane. Nanomolar working concentrations are enough to label live cells effectively, and multiple colours exist across the family so samples can be matched to multi-label designs. Typical use is following lysosomal biosynthesis and related disease mechanisms. |
| Intended Use | Selective labelling of acidic organelles in live cells for lysosome biology, biosynthesis tracking and related pathology studies. |
| Principle / Technology | The fluorophore-linked weak base permeates the cell membrane and accumulates in acidic organelles through protonation and membrane retention. |
| Detection Method | Blue fluorescence at Ex 373 nm / Em 422 nm by fluorescence microscopy. |
| Sample Type | Live cultured cells containing lysosomes. |
| Performance Range / Specifications | CAS 215247-93-1; molecular formula C24H38Cl4N4; molecular weight 534.4 g/mol; appearance colourless solution; Ex/Em 373/422 nm; effective at nanomolar concentrations; supplied as 1 mM stock in anhydrous DMSO. |
| Sensitivity / LOD | Effective labelling at nanomolar working levels; no numeric detection limit is quoted. |
| Specificity | Selective for acidic organelles rather than the neutral cytoplasm; blue channel suited to multi-colour designs. |
| Reaction Conditions / Protocol | Dilute the 1 mM DMSO stock to a nanomolar working solution, incubate live cells, wash gently if required, then image on the blue channel. |
| Components / Formulation | One vial of 50 μL probe solution (1 mM in anhydrous DMSO). |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 50 μL per vial |
| Product Form | Colourless solution in anhydrous DMSO. |
| Key Features | - Selective acidic-organelle labelling in live cells - Effective at nanomolar working concentrations - Blue 373/422 nm read-out for multi-colour designs - Simple incubation protocol - Supplied as a 1 mM anhydrous DMSO stock |
| Concentration | 1 mM stock solution in anhydrous DMSO. |
| Activity / Unit Definition | Probe performance is demonstrated by selective nanomolar labelling of lysosomes. |
| Molecular Weight | CAS 215247-93-1; molecular formula C24H38Cl4N4; molecular weight 534.4 g/mol. |
| Source / Origin | Synthetic small-molecule fluorescent probe, supplied as a DMSO stock. |
| Expiration Date / Stability | Stable through the stated shelf life when kept protected from light at the recommended storage temperature. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with standard fluorescence instrumentation - microscopes, plate readers, flow cytometers or imaging systems as appropriate to the format. |
| Recommended Buffer System | Supplied in anhydrous DMSO as a 1 mM stock; working solutions are prepared by the user. |
| Application Notes / Precautions | Handle protected from light, minimise freeze-thaw cycles by preparing aliquots, and verify that the detection channel matches the dye before use. |
| Batch-to-Batch Consistency | Consistency between lots is maintained by fixed production controls and release testing against specification. |
| Model Type | Organelle-selective fluorescent probe (lysosome) |
| Starting Material / Tissue Site | Synthesised acidotropic weak-base fluorophore |
| Delivery Standard | 50 μL vial at 1 mM |
| Readouts / Downstream Assays | Blue fluorescence at Ex 373/Em 422 nm marking acidic organelles. |
For research use only, not for clinical use.
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