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| Product Name | Lysosomal pH Yellow/Blue Fluorescent Probe (DND-160) |
| Catalog No. | RESK-0559 |
| Description | This series of probes comprises fluorescent probes for measuring the pH of acidic compartments (such as lysosomes and sperm acrosomes) in living cells. The probes are acidotropic: they accumulate in acidic organelles through protonation, and this acidotropism relieves fluorescence quenching of the dye, resulting in stronger emitted fluorescence. Therefore, unlike pH-independent lysosomal probes (such as the DND-26/DND-99 type), these pH-sensitive probes show a pH-dependent (acid-directed) increase in fluorescence intensity as the organelle acidifies. The Lysosomal pH Yellow/Blue Fluorescent Probe (DND-160, PDMPO) is a ratiometric probe for measuring lysosomal pH. It is a pH-dependent fluorescent probe displaying dual excitation and dual emission wavelengths: it produces blue fluorescence in neutral environments (Ex/Em = 329 nm/440 nm) and emits yellow fluorescence when the environment becomes acidic (Ex/Em = 384 nm/540 nm). The product is supplied as a 1 mM stock solution in anhydrous DMSO. |
| Intended Use | Ratiometric measurement of pH in acidic compartments (e.g., lysosomes, sperm acrosomes) of living cells. |
| Principle / Technology | The acidotropic probe accumulates in acidic organelles via protonation, which relieves fluorescence quenching and strengthens the emitted fluorescence in a pH-dependent manner. DND-160 (PDMPO) exhibits dual excitation and dual emission: blue fluorescence (Ex/Em = 329 nm/440 nm) at neutral pH shifts to yellow fluorescence (Ex/Em = 384 nm/540 nm) under acidic conditions, enabling ratiometric pH measurement. |
| Detection Method | Ratiometric fluorescence detection with dual excitation (329 nm and 384 nm) and dual emission (440 nm and 540 nm). |
| Sample Type | Living cells (acidic compartments such as lysosomes and sperm acrosomes). |
| Performance Range / Specifications | Molecular formula: C20H22N4O3 Molecular weight: 366.4188 Ex: 329 nm, 384 nm; Em: 440 nm, 540 nm pKa: ~4.2 Applicable pH detection range: 3.5-6.0 Appearance: colorless solution |
| Specificity | pH-dependent, acidotropic probe that accumulates in acidic organelles of living cells for ratiometric lysosomal pH determination. |
| Components / Formulation | Lysosomal pH Yellow/Blue Fluorescent Probe (DND-160, PDMPO), supplied as a 1 mM stock solution in anhydrous DMSO; colorless solution; non-sterile. |
| Storage Conditions | Store at -20 °C, protected from light and dry; avoid repeated freeze-thaw cycles. |
| Shelf Life | One year when stored at -20 °C, protected from light. |
| Package Specifications | 50 μL |
| Product Form | Colorless solution (1 mM stock in anhydrous DMSO) |
| Concentration | 1 mM stock solution in anhydrous DMSO |
| Molecular Weight | 366.4188 |
| pH Range / Optimal pH | Applicable pH detection range: 3.5-6.0; pKa ~4.2 |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for one year when stored at -20 °C, protected from light and dry; avoid repeated freeze-thaw cycles. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | The product is non-sterile. The stock solution is colorless. Blue fluorescence (Ex/Em = 329 nm/440 nm) is produced at neutral pH; yellow fluorescence (Ex/Em = 384 nm/540 nm) is emitted as the environment becomes acidic. |
For research use only, not for clinical use.
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