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| Product Name | Lysosomal pH Blue Fluorescent Probe (Ex/Em 373/425 nm, pKa about 5.1) |
| Catalog No. | RERE-0014 |
| Description | This blue probe belongs to the pH-sensing family of acidotropic dyes. It accumulates in acidic organelles such as lysosomes through protonation, and unlike simple labelling dyes its fluorescence strengthens as the compartment acidifies, rising in a pH-dependent manner. That behaviour lets it measure the pH of lysosomes and similar compartments in live cells rather than merely marking them. The probe is supplied as a 1 mM stock in anhydrous DMSO; if selective labelling of acidic compartments without pH read-out is the goal, a dedicated lysosome tracer dye is the better choice. |
| Intended Use | Measurement of pH in lysosomes and other acidic organelles of live cells, with signal that increases as acidity rises. |
| Principle / Technology | Acidotropic accumulation: the weak base enters acidic organelles and is protonated, which both traps it there and relieves fluorescence quenching, so emission rises as pH falls. |
| Detection Method | Blue fluorescence at Ex 373 nm / Em 425 nm by fluorescence microscopy. |
| Sample Type | Live cultured cells containing acidic organelles. |
| Performance Range / Specifications | Molecular formula C24H28N2O2; molecular weight 376.5 g/mol; pKa about 5.1; appearance colourless solution; Ex 373 nm / Em 425 nm; supplied as 1 mM stock in anhydrous DMSO. |
| Specificity | pH-responsive acidotropic behaviour with a pKa near 5.1, matched to the acidity of lysosomal compartments. |
| Reaction Conditions / Protocol | Dilute the 1 mM DMSO stock into the working solution, incubate live cells, then record the blue signal and interpret it against pH standards. |
| Components / Formulation | One vial of 50 μL probe solution (1 mM in anhydrous DMSO). |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 50 μL per vial |
| Product Form | Colourless solution in anhydrous DMSO. |
| Key Features | - Fluorescence rises with organelle acidification - pKa about 5.1 matched to lysosomal acidity - Measures pH rather than only labelling - Blue 373/425 nm read-out - Supplied as a 1 mM anhydrous DMSO stock |
| Concentration | 1 mM stock solution in anhydrous DMSO. |
| Activity / Unit Definition | Probe performance is anchored by its pH-dependent fluorescence behaviour around the documented pKa. |
| Molecular Weight | Molecular formula C24H28N2O2; molecular weight 376.5 g/mol |
| Source / Origin | Synthetic small-molecule fluorescent probe, supplied as a DMSO stock. |
| Expiration Date / Stability | Stability is maintained by the recommended storage temperature together with continuous protection from light. |
| Regulatory / Compliance | Supplied for laboratory research purposes. |
| Compatibility | Read-outs run on routine fluorescence equipment without proprietary hardware. |
| Recommended Buffer System | Supplied in anhydrous DMSO as a 1 mM stock; working solutions are prepared by the user. |
| Application Notes / Precautions | Protect from light at all times, both in storage and during labelling, and avoid repeated warming cycles. Prepare stock solutions fresh where possible and confirm the instrument filter set before starting an experiment. |
| Batch-to-Batch Consistency | Each production lot is released against fixed acceptance criteria, keeping performance consistent between orders. |
| Model Type | Organelle pH-sensing fluorescent probe (lysosome/acidic compartments) |
| Starting Material / Tissue Site | Synthesised acidotropic fluorophore with pH-dependent emission |
| Delivery Standard | 50 μL vial at 1 mM |
| Turnaround Time | dispatch timing is settled at purchase. |
| Readouts / Downstream Assays | Blue fluorescence at Ex 373/Em 425 nm rising with organelle acidification. |
For research use only, not for clinical use.
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