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| Product Name | Luminol |
| Catalog No. | RERE-0049 |
| Description | Luminol is a chemiluminescent reagent that oxidises readily in alkaline solution and releases the stored energy as visible light. The oxidation route depends on the medium: in aprotic solvents such as DMSO or DMF molecular oxygen and a strong base alone trigger emission centred near 485 nm, whereas in water a strong base together with molecular oxygen or hydrogen peroxide and a cooxidant such as hypochlorite or perborate is required, with emission near 425 nm. The emitting species is the excited state of 3-aminophthalate. Applications span enzymatic determination of glucose and glucose oxidase, use as a poly(ADP-ribose) polymerase inhibitor, chemiluminescence demonstrations with different sensitisers, forensic detection of trace blood and biochemical detection of metal cations such as copper, iron and cyanide, alongside horseradish peroxidase coupled Western Blot readouts. |
| Intended Use | Chemiluminescent analysis, enzyme coupled glucose and glucose oxidase determination, PARP inhibition studies, chemiluminescence demonstrations, forensic trace blood examination, metal cation detection and Western Blot luminescent readouts. |
| Principle / Technology | Oxidation of luminol in alkaline medium populates the excited state of 3-aminophthalate, which decays with emission of visible blue light proportional to analyte or enzyme activity. |
| Detection Method | Peroxidase and oxidase enzyme activities; reactive oxidants and metal cations |
| Performance Range / Specifications | Emission maximum 425 nm in aqueous systems and 485 nm in aprotic solvents. |
| Specificity | Chemiluminescence arises specifically from oxidation of the luminol molecule. |
| Storage Conditions | 2 to 8 °C, protected from light in a cool place |
| Shelf Life | 1 year |
| Package Specifications | 1 g; 5 g |
| Product Form | Off-white to pale yellow powder |
| Quality Control | Purity at 98% or higher by HPLC; loss on drying no more than 8.0%. |
| Key Features | High purity HPLC grade material; versatile across enzyme assays, demonstrations and forensic work; stable powder format. |
| Purity | >=98% (HPLC) |
| Molecular Weight | 177.16 |
| Shipping Conditions | Cold pack |
| Regulatory / Compliance | For research use. |
| Compatibility | Soluble in DMSO (50 mg/mL), readily soluble in alkaline solution, soluble in hot acetic acid, ethanol and ether, nearly insoluble in water. |
| Batch-to-Batch Consistency | Lot specific certificate of analysis. |
For research use only, not for clinical use.
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