- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Live/Dead Fixable Violet Dead Cell Discrimination Stain |
| Catalog No. | CCAT-HMM-0058 |
| Description | An amine-reactive fluorescent viability dye designed for discrimination of dead cells in flow cytometry experiments requiring subsequent fixation and permeabilization. The dye reacts with free amines on both the cell surface and intracellular proteins. In live cells with intact membranes, only surface amines are labeled, resulting in dim staining. In dead cells with compromised membrane integrity, both surface and abundant intracellular amines are labeled, producing bright fluorescence that persists through fixation and permeabilization procedures. |
| Intended Use | Irreversible discrimination of live and dead cells for flow cytometry experiments incorporating intracellular staining protocols. |
| Principle / Technology | Amine-reactive fluorescent dye with differential membrane permeability-based staining intensity |
| Detection Method | Flow cytometry (violet laser excitation, 405 nm; emission 450/50 nm) |
| Sample Type | Single cell suspensions from any tissue or culture source; compatible with human, mouse, and other mammalian cells |
| Performance Range / Specifications | Staining: 30 min at room temperature; fluorescent signal maintained after formaldehyde fixation and methanol permeabilization |
| Sensitivity / LOD | Clear separation between live and dead cell populations with signal-to-noise ratio ≥100:1 |
| Specificity | Amine-reactive dye binds all cellular amines; discrimination based on differential accessibility rather than target specificity |
| Reaction Conditions / Protocol | Wash cells in protein-free PBS; resuspend at 1 × 10⁶/mL; add 1 µL dye per mL cell suspension; incubate 30 min at room temperature protected from light; wash; proceed to surface staining or fixation |
| Components / Formulation | Fixable Violet Viability Dye (lyophilized or DMSO stock), anhydrous DMSO for reconstitution |
| Storage Conditions | Lyophilized: –20 °C desiccated; reconstituted: –20 °C single-use aliquots, protected from light |
| Shelf Life | 12 months lyophilized; 1 month reconstituted |
| Package Specifications | 100 tests or 500 tests |
| Product Form | Lyophilized dye or DMSO solution |
| Quality Control | Each lot verified by staining a 1:1 mixture of heat-killed and live lymphocytes; dead cell population mean fluorescence intensity ≥100× live population |
| Key Features | Covalent binding withstands fixation and permeabilization, maintaining dead cell discrimination through intracellular staining steps |
| Purity | Reagent grade; fluorophore-labeled reagents ≥90% labeling efficiency |
| Concentration | As specified per kit; sufficient for stated number of tests |
| Activity / Unit Definition | Apoptosis induction confirmed by positive control treatment producing ≥5-fold signal increase |
| Molecular Weight | Varies by dye and enzyme component |
| Source / Origin | Recombinant annexin proteins; synthetic fluorophore conjugates; chromogenic/fluorogenic peptide substrates |
| pH Range / Optimal pH | Binding/washing buffer pH 7.2–7.4 |
| Shipping Conditions | Cold packs; protect fluorophores from light; some components may require –20 °C |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 |
| Compatibility | Compatible with flow cytometry and fluorescence microscopy platforms |
| Recommended Buffer System | Annexin binding buffer with calcium; cell lysis buffer with protease inhibitors for caspase assays |
| Application Notes / Precautions | Process samples promptly after staining; include unstained and single-stain controls for flow cytometry compensation; use appropriate apoptosis inducers as positive controls |
| Batch-to-Batch Consistency | Positive control signal within ±20% of reference lot; staining pattern consistent |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
