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LAMP Fluorescent Dye

Cat.No: ATRSA-0036 Datasheet

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Product Details Related Products
Product Name LAMP Fluorescent Dye
Catalog No. ATRSA-0036
Description A concentrated nucleic acid intercalating dye that provides strong fluorescence with limited inhibition in real-time LAMP assays.
Intended Use Real-time fluorescent monitoring of LAMP and RT-LAMP amplification.
Principle / Technology The dye intercalates into amplified nucleic acid and produces a fluorescence signal as product accumulates.
Detection Method SYBR/FAM fluorescence channel.
Sample Type LAMP or RT-LAMP reaction mixtures.
Performance Range / Specifications 50× solution; recommended final concentration 0.1× to 1×; package volume 100 µL.
Specificity Designed to minimize reaction inhibition while providing a high fluorescence plateau.
Reaction Conditions / Protocol Add the dye to the amplification reaction at a final concentration of 0.1× to 1× and monitor fluorescence through the SYBR/FAM channel.
Components / Formulation 50× fluorescent nucleic acid dye in DMSO.
Package Specifications 100 µL.
Product Form 50× fluorescent dye solution.
Quality Control Fluorescence profile, amplification inhibition, freeze–thaw stability, light stability and instrument compatibility are evaluated.
Key Features Strong fluorescence; high signal plateau; low reaction inhibition; broad real-time PCR instrument compatibility; good storage stability.
Concentration 50× stock solution.
Compatibility Compatible with common real-time PCR instruments using the SYBR/FAM channel.
Application Notes / Precautions Protect from light and optimize the final dye concentration for the selected instrument and assay chemistry.

For research use only, not for clinical use.

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