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| Product Name | Lactophenol Trypan Blue Staining Solution, 0.05% |
| Catalog No. | RERE-0292 |
| Description | Specialty stain built from lactic acid, glycerol, trypan blue and water at a 0.05 percent dye level, formulated for observing filamentous fungi. It is also the standard reagent for measuring arbuscular mycorrhizal colonization rates, where root fungal structures pick up the blue dye against cleared plant tissue. The lactophenol base simultaneously stains, preserves and mounts the specimen. Shelf life is 12 months at room temperature away from light. |
| Intended Use | Staining of fungi and quantification of arbuscular mycorrhizal colonization in roots |
| Principle / Technology | Trypan blue binds fungal chitinous structures while the lactophenol-glycerol vehicle clears plant tissue and fixes the preparation |
| Specificity | 0.05 percent; lactic acid, glycerol, trypan blue and water; suited to mycorrhizal colonization studies; 12 month shelf life |
| Reaction Conditions / Protocol | Clear and soften the root sample, stain with the solution, destain as required and mount for microscopic scoring |
| Storage Conditions | Room temperature, protected from light |
| Package Specifications | 50 mL / 100 mL |
| Key Features | Combined staining and mounting vehicle; established reagent for mycorrhizal colonization work; 12 month shelf life |
For research use only, not for clinical use.
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