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| Product Name | JC-10 Mitochondrial Membrane Potential Fluorescent Probe |
| Catalog No. | RESK-0523 |
| Description | JC-10 is an upgraded alternative to JC-1 for monitoring mitochondrial membrane potential. Although JC-1 is widely used, its poor water solubility causes precipitation in aqueous buffers even at 1 µM, whereas JC-10 offers markedly better water solubility and can replace JC-1 in assays that require higher dye concentrations. In healthy cells, JC-10 selectively accumulates in the mitochondrial matrix and forms reversible red-fluorescent J-aggregates (Ex = 540 nm, Em = 590 nm). In unhealthy mitochondria with reduced or lost membrane potential, JC-10 remains in the cytoplasm as green-fluorescent monomers (Ex = 490 nm, Em = 525 nm). The dye supports both qualitative analysis, since the color shift directly reflects changes in mitochondrial membrane potential, and quantitative analysis, since the degree of mitochondrial depolarization can be measured by the red/green fluorescence intensity ratio. The two signals are detected with standard flow cytometer filters: green fluorescence in the FL1 channel and red fluorescence in the FL2 channel. In addition to flow cytometry, JC-10 is suitable for fluorescence imaging and fluorescence microplate reader platforms. In some cell lines JC-10 performs better than JC-1; however, its performance is highly cell-type dependent. |
| Intended Use | Detection and quantification of changes in mitochondrial membrane potential in cell-based assays. For research use only. |
| Principle / Technology | Membrane-potential-dependent aggregation: JC-10 accumulates in energized mitochondria and forms red-fluorescent J-aggregates (Ex/Em = 540/590 nm); upon loss of membrane potential it reverts to green-fluorescent monomers (Ex/Em = 490/525 nm) in the cytoplasm. The red/green fluorescence ratio reflects the degree of mitochondrial depolarization. |
| Detection Method | Flow cytometry (green fluorescence in FL1, red fluorescence in FL2) Fluorescence imaging Fluorescence microplate reader |
| Sample Type | Cultured cells |
| Performance Range / Specifications | Chemical name: 5,6-Dichloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide; Enhanced JC-1 Formula: C25H27Cl2IN4 Molecular weight: 583.34 Purity: >95% (HPLC) Appearance: red powder Solubility: soluble in DMSO Fluorescence spectra: monomer form Ex = 490 nm, Em = 525 nm; J-aggregate form Ex = 540 nm, Em = 590 nm |
| Specificity | Selectively accumulates in the mitochondrial matrix of healthy, polarized mitochondria. Performance is highly cell-type dependent. |
| Components / Formulation | Supplied as a solid dye; prepare stock solutions in DMSO. |
| Storage Conditions | Powder: store at -20°C, dry and protected from light. Stock solution: aliquot into single-use portions and store at -20°C, dry and protected from light; avoid repeated freeze-thaw cycles. |
| Shelf Life | Powder: at least one year at -20°C. Stock solution: approximately one year at -20°C. |
| Package Specifications | 1 mg 5 mg |
| Product Form | Red powder |
| Quality Control | Purity verified by HPLC (>95%). |
| Purity | >95% (HPLC) |
| Molecular Weight | 583.34 |
| Shipping Conditions | Shipped on wet ice. |
| Expiration Date / Stability | Stable for at least one year when stored dry at -20°C and protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with standard flow cytometer filter sets (FL1 for green, FL2 for red), fluorescence imaging systems, and fluorescence microplate readers. |
| Recommended Buffer System | Aqueous physiological buffers for working solutions; stock solutions prepared in DMSO. |
| Application Notes / Precautions | JC-10 performance is highly cell-type dependent; in some cell lines it performs better than JC-1. Thanks to its improved water solubility, JC-10 can replace JC-1 in assays requiring higher dye concentrations. Protect from light and avoid repeated freeze-thaw cycles of stock solutions. |
For research use only, not for clinical use.
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