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| Product Name | JC-1 Mitochondrial Membrane Potential Fluorescent Probe |
| Catalog No. | RESK-0522 |
| Description | JC-1 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential (delta psi m). The JC-1 dye accumulates in mitochondria in a potential-dependent manner and can be used to measure the membrane potential of cells, tissues or purified mitochondria. In healthy mitochondria, JC-1 aggregates in the mitochondrial matrix to form J-aggregates that emit intense red fluorescence (Ex = 585 nm, Em = 590 nm). In unhealthy mitochondria, where the membrane potential is decreased or lost, JC-1 exists only as monomers in the cytosol, producing green fluorescence (Ex = 514 nm, Em = 529 nm). JC-1 can be used not only for qualitative detection, because the color change directly reflects changes in mitochondrial membrane potential, but also for quantitative detection, because the degree of mitochondrial depolarization is measured by the ratio of red to green fluorescence intensity. Only conventional red and green fluorescence observation settings are needed. For detecting the mitochondrial membrane potential of cells, the commonly used JC-1 concentration range is 1-20 μg/mL, and 10 μg/mL is suitable for many cell types. |
| Intended Use | Qualitative and quantitative detection of mitochondrial membrane potential (delta psi m) in cells, tissues or purified mitochondria. |
| Principle / Technology | JC-1 accumulates in mitochondria in a potential-dependent manner. At high membrane potential it forms red-fluorescent J-aggregates in the mitochondrial matrix; when the membrane potential decreases or is lost, it remains as green-fluorescent monomers in the cytosol. The red/green fluorescence intensity ratio quantifies the degree of mitochondrial depolarization. |
| Detection Method | Fluorescence detection with conventional red and green fluorescence settings: J-aggregate (red) Ex = 585 nm, Em = 590 nm; monomer (green) Ex = 514 nm, Em = 529 nm. Qualitative readout by color change; quantitative readout by the red/green fluorescence ratio. |
| Sample Type | Living cells, tissues (digested into single viable cells before staining) or purified mitochondria. |
| Performance Range / Specifications | Chemical name: 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide CAS No.: 3520-43-2 Formula: C25H27Cl4IN4 Molecular weight: 652.23 Purity: > 95% (HPLC) Appearance: red-brown powder Solubility: soluble in DMSO and DMF; insoluble in water Fluorescent spectrum: monomer form Ex = 514 nm, Em = 529 nm; J-aggregate form Ex = 585 nm, Em = 590 nm |
| Storage Conditions | Powder: store dry at -20 °C, protected from light. Stock solution: aliquot into single-use portions and store dry at -20 °C, protected from light; avoid repeated freeze-thaw cycles. |
| Shelf Life | Powder: at least 1 year at -20 °C (dry, protected from light). Stock solution: approximately 1 year at -20 °C when aliquoted into single-use portions and protected from light. |
| Package Specifications | 1 mg, 5 mg |
| Product Form | Red-brown powder; soluble in DMSO and DMF, insoluble in water. |
| Quality Control | Purity > 95% as determined by HPLC. |
| Purity | > 95% (HPLC) |
| Concentration | Recommended working concentration: 1-20 μg/mL; 10 μg/mL is suitable for many cell types. |
| Molecular Weight | 652.23 |
| Shipping Conditions | Shipped on wet ice. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | For tissue section samples, digest the living tissue into single viable cells before staining. Do not fix cells before staining: JC-1 accumulates in mitochondria in a potential-dependent manner, and fixation destroys the membrane potential so that the probe can no longer accumulate in mitochondria and loses specificity. If aggregated particles appear in the JC-1 working solution, centrifuge at 13,000 g for 1-2 minutes to remove them before staining. |
For research use only, not for clinical use.
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