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| Product Name | Immobilon-P PVDF Transfer Membrane (0.45 μm) |
| Catalog No. | PTR-HMM-0080 |
| Description | A polyvinylidene difluoride membrane with 0.45 μm pore size optimized for Western blot protein transfer from SDS-PAGE gels. The membrane provides high protein binding capacity and mechanical strength for immunoblot detection workflows. |
| Intended Use | Protein immobilization following SDS-PAGE for subsequent immunodetection by Western blotting using chemiluminescent, fluorescent, or chromogenic detection methods. |
| Principle / Technology | Proteins are electrophoretically transferred from the polyacrylamide gel matrix onto the PVDF membrane, where they bind through hydrophobic and dipole interactions, creating a replica of the gel's protein separation pattern. |
| Detection Method | Immunodetection with primary and secondary antibodies followed by ECL, fluorescent, or chromogenic visualization |
| Sample Type | Proteins separated by SDS-PAGE or native PAGE |
| Performance Range / Specifications | Protein binding capacity: 125–200 μg/cm² for IgG; pore size: 0.45 μm; applicable molecular weight range: >20 kDa; membrane dimensions: 10 cm × 15 cm sheets or 26.5 cm × 3.75 m roll |
| Sensitivity / LOD | Detection sensitivity commensurate with the immunodetection system; typical detection limits in the low picogram range with optimized antibodies and ECL detection |
| Specificity | Binds most proteins regardless of isoelectric point; hydrophobic PVDF surface provides strong protein retention during washing and reprobing cycles |
| Reaction Conditions / Protocol | Pre-wet membrane in 100% methanol for 15 seconds, rinse in ultrapure water for 2 minutes, equilibrate in transfer buffer for at least 5 minutes, assemble transfer sandwich, transfer at 100V for 60 minutes (wet transfer) or according to semi-dry transfer protocol |
| Components / Formulation | PVDF membrane sheets or rolls, hydrophobic surface requires methanol pre-wetting before aqueous buffer equilibration |
| Storage Conditions | Room temperature; store in a dry environment; membrane sheets should remain in the protective interleaf paper until use |
| Shelf Life | 36 months from date of manufacture |
| Package Specifications | 10 sheets (10 cm × 15 cm) or 1 roll (26.5 cm × 3.75 m) |
| Product Form | Flat PVDF membrane sheets or roll with interleaf paper |
| Quality Control | Each lot tested for protein binding capacity using 125I-labeled IgG; wetting uniformity verified; background signal tested with standard ECL detection system |
| Key Features | PVDF provides superior protein retention compared to nitrocellulose, enabling multiple stripping and reprobing cycles on a single membrane; the high mechanical strength allows convenient handling without tearing; 0.45 μm pore size provides optimal balance of binding capacity and flow characteristics |
For research use only, not for clinical use.
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