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| Product Name | HRP-Conjugated Goat Anti-Mouse IgG Secondary Antibody |
| Catalog No. | PTR-HMM-0065 |
| Description | A horseradish peroxidase (HRP)-conjugated goat polyclonal secondary antibody specific for mouse IgG heavy and light chains. This reagent is optimized for Western blot, ELISA, and immunohistochemistry detection applications. |
| Intended Use | Detection of mouse primary antibodies in immunoassays including Western blotting, direct and indirect ELISA, and immunohistochemical staining of tissue sections. |
| Principle / Technology | Goat-derived polyclonal antibodies raised against mouse IgG heavy and light chains, affinity-purified and conjugated to horseradish peroxidase for enzymatic signal amplification. |
| Detection Method | Colorimetric, chemiluminescent, or fluorescent detection depending on HRP substrate selection |
| Sample Type | Mouse primary antibodies bound to target antigens in membrane, microplate, or tissue section formats |
| Performance Range / Specifications | Recommended working dilution: 1:5,000–1:50,000 for Western blot; 1:10,000–1:100,000 for ELISA; titer should be optimized per application |
| Sensitivity / LOD | Detection of primary antibody at concentrations as low as 1 ng/mL depending on substrate sensitivity and primary antibody affinity |
| Specificity | Reacts with mouse IgG heavy and light chains; exhibits cross-adsorption against human, bovine, and horse serum proteins to minimize non-specific binding |
| Reaction Conditions / Protocol | Dilute antibody in appropriate blocking buffer, incubate with sample for 1 hour at room temperature (or overnight at 4°C), wash extensively, detect with HRP substrate |
| Components / Formulation | Purified HRP-conjugated goat anti-mouse IgG (H+L) antibody at 1 mg/mL in PBS with 50% glycerol, 0.1% BSA as stabilizer, 0.01% thimerosal preservative |
| Storage Conditions | -20°C for long-term storage; 2–8°C for short-term working aliquots (up to 4 weeks); avoid repeated freeze-thaw cycles |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 1 mg (1 mL at 1 mg/mL) |
| Product Form | Liquid antibody-glycerol solution |
| Quality Control | Each lot tested by direct ELISA for minimum titer (≥1:100,000 dilution producing OD >1.0); Western blot validation with standard protein lysates; HRP enzyme activity verified (>200 U/mg) |
| Key Features | Affinity-purified for minimal background; cross-adsorbed against multiple species for reduced cross-reactivity; HRP conjugation provides versatile detection with multiple substrate options |
For research use only, not for clinical use.
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