- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Hoechst 33342 |
| Catalog No. | RESK-0540 |
| Description | Hoechst 33342 is a cell membrane-permeable blue fluorescent dye that emits strong blue fluorescence upon binding to double-stranded DNA and shows low cytotoxicity to cells. It is commonly used for apoptosis detection, with stained cells examined by fluorescence microscopy or analyzed by flow cytometry, and is also suitable for routine nuclear counterstaining and general DNA staining. This product is supplied as a powder. For nuclear staining, the recommended working concentration of Hoechst 33342 is 0.5-10 μg/mL. A ready-to-use 1 mg/mL stain solution is also available as a separate product. |
| Intended Use | Qualitative and quantitative fluorescence staining of nuclear DNA in live or fixed cells; apoptosis detection; routine nuclear counterstaining and general DNA staining, analyzed by fluorescence microscopy or flow cytometry. |
| Principle / Technology | Hoechst 33342 is a bisbenzimide dye that crosses intact cell membranes and binds to double-stranded DNA. Upon DNA binding its blue fluorescence is strongly enhanced. Unlike DAPI, which is semi-permeant to impermeant and gives inconsistent labeling of live cells, Hoechst 33342 is fully membrane-permeant and is therefore well suited for live-cell imaging, while performing like DAPI on fixed samples. |
| Detection Method | Fluorescence microscopy; flow cytometry |
| Sample Type | Live cells; fixed and permeabilized cells or tissues |
| Performance Range / Specifications | English synonym: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole, trihydrochloride Molecular formula: C27H28N6O·3HCl Molecular weight: 561.93 CAS No.: 23491-52-3 Fluorescence spectra: Ex/Em = 346/460 nm for free Hoechst 33342; Ex/Em = 350/461 nm for the Hoechst 33342-nucleic acid complex Purity: ≥95% (HPLC) |
| Specificity | Selectively binds double-stranded DNA with low cytotoxicity; suitable for live-cell nuclear labeling. |
| Reaction Conditions / Protocol | For nuclear staining, prepare a working solution at 0.5-10 μg/mL in an appropriate buffer and incubate cells before analysis by fluorescence microscopy or flow cytometry. Live cells can be stained directly without fixation. Staining in PBS buffer generally gives better results than serum-free medium; when staining in serum-free medium gives weak fluorescence or requires prolonged incubation, switching to PBS is recommended. |
| Components / Formulation | Hoechst 33342 powder, ≥95% purity (HPLC) |
| Storage Conditions | Store at -20°C in a dry place, protected from light. |
| Shelf Life | 2 years when stored at -20°C, dry and protected from light. |
| Package Specifications | 10 mg |
| Product Form | Powder |
| Purity | ≥95% (HPLC) |
| Molecular Weight | 561.93 |
| Shipping Conditions | Shipped on wet ice. |
| Expiration Date / Stability | Stable for 2 years at -20°C when kept dry and protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Fluorescence can be excited with a xenon lamp, mercury arc lamp, or UV laser, and detected using a DAPI filter set or a blue GFP filter set. Can be combined with lysosomal probes for simultaneous staining of the nucleus and lysosomes in live cells. |
| Recommended Buffer System | PBS buffer is recommended for staining; serum-free medium may give weak fluorescence and prolonged staining times. |
| Application Notes / Precautions | Recommended working concentration for nuclear staining: 0.5-10 μg/mL. Protect from light during storage and handling. Staining in PBS buffer gives better results than serum-free medium. For simultaneous lysosome and nucleus staining in live cells, this dye can be used directly without fixation. |
| Recognition Site | Double-stranded DNA (nuclear DNA) |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
