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| Product Name | High-sensitivity acridinium salt NSP-SA-NHS for biomolecule detection |
| Catalog No. | IGM-HMM-0171 |
| Description | Acridinium salt NSP-SA-NHS exhibits enhanced hydrophilicity and reactivity. It is highly soluble in aqueous solution and reacts with molecules bearing amino or thiol groups to form luminescent conjugates, a property that gives it broad utility in fluorescence analysis and detection. Beyond analytical labeling, it can be used to construct high-sensitivity fluorescent probes and biosensors capable of detecting biomolecules, ions and temperature, providing effective tools for biological research, environmental monitoring and chemical analysis. Owing to its high sensitivity, high stability and high reactivity, NSP-SA-NHS is widely applied in chemiluminescent immunoassay, bioanalysis, environmental monitoring and food safety testing. |
| Intended Use | High-sensitivity chemiluminescent labeling reagent for the detection of biological substances, and for the preparation of fluorescent probes and biosensors used in research, environmental monitoring and food safety analysis. |
| Principle / Technology | As a chemiluminescent label, NSP-SA-NHS undergoes light emission in alkaline hydrogen peroxide solution. The peroxide anion attacks the acridinium ester to generate a strained, unstable dioxetane intermediate, which decomposes into CO2 and an electronically excited acridone. When the acridone returns to its ground state, it emits a photon at an absorption wavelength of 430 nm. The entire flash is extremely brief, completing in under 2 seconds, so a fast-response detection system is required. |
| Detection Method | Chemiluminescence (430 nm flash emission in alkaline hydrogen peroxide); also applicable in fluorescence-based analysis. |
| Reaction Conditions / Protocol | Labeling proceeds under alkaline conditions with proteins containing primary amino groups. The NHS moiety acts as a leaving group and is displaced, allowing the protein to form a stable amide bond with the acridinium ester. After the reaction, excess acridinium salt is removed using a desalting column. In the presence of alkaline hydrogen peroxide, the acridinium-labeled protein emits light on its own, without any enzymatic catalysis. |
| Package Specifications | 10 mg/vial; 1 g/vial |
| Key Features | Enhanced hydrophilicity and high aqueous solubility High sensitivity, high stability and high reactivity Reacts with amino- or thiol-bearing molecules to form luminescent conjugates Direct, enzyme-free light emission enabling rapid detection |
| Purity | >98% |
| Application Notes / Precautions | Chemiluminescent immunoassay and bioanalysis Environmental monitoring and food safety testing Construction of high-sensitivity fluorescent probes and biosensors for biomolecules, ions and temperature |
| CAS Number | 199293-83-9 |
| Other Name | NSP-SA-NHS |
| Functional Group | NHS (N-hydroxysuccinimide) ester, reactive toward primary amines |
For research use only, not for clinical use.
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