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| Product Name | High-Efficiency RT SuperMix for qPCR with gDNA Removal |
| Catalog No. | NATR-HMM-0173 |
| Description | A high-efficiency qPCR reverse-transcription system with a rapid genomic DNA removal module for difficult, low-concentration and degraded RNA samples. |
| Intended Use | First-strand cDNA synthesis; reverse transcription; gene expression analysis; two-step RT-qPCR. |
| Principle / Technology | An upgraded reverse transcriptase and optimized buffer convert RNA into cDNA after rapid genomic DNA removal. |
| Sample Type | Total RNA, including low-concentration and degraded RNA such as FFPE-derived RNA. |
| Performance Range / Specifications | 100 reactions; supports RNA input up to 5 µg; rapid genomic DNA removal in approximately 2 minutes; suitable for low-expression and degraded RNA. |
| Specificity | Designed to improve reverse-transcription efficiency and qPCR sensitivity with challenging RNA templates. |
| Reaction Conditions / Protocol | Perform the genomic DNA removal step for approximately 2 minutes, then complete reverse transcription according to the protocol. |
| Components / Formulation | High-efficiency RT supermix and genomic DNA removal module. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions. |
| Product Form | qPCR RT supermix with genomic DNA removal module. |
| Quality Control | Evaluated with low-concentration and degraded RNA, including highly degraded cell RNA and FFPE-derived RNA. |
| Key Features | High reverse-transcription efficiency; broad template compatibility; rapid genomic DNA removal; suitable for degraded RNA and low-expression targets. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Resulting cDNA is compatible with dye-based and probe-based qPCR. |
| Application Notes / Precautions | Mix glycerol-containing reagents thoroughly before use. If genomic DNA remains, the removal step may be extended for up to 15 minutes. The system is optimized for qPCR and generally produces cDNA targets below approximately 550 bp rather than long amplicons. |
For research use only, not for clinical use.
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