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| Product Name | High-Efficiency Inhibitor-Tolerant Reverse Transcriptase |
| Catalog No. | IEC-HMM-0102 |
| Description | A high-efficiency reverse transcriptase with broad template compatibility, strong processivity and resistance to common RNA-extraction contaminants. |
| Intended Use | Reverse transcription; first-strand cDNA synthesis; two-step RT-qPCR; cDNA library construction. |
| Principle / Technology | An engineered reverse transcriptase performs efficient cDNA synthesis at 37°C and retains the thermal stability needed for 50–55°C reactions with structured RNA templates. |
| Detection Method | Used upstream of PCR, qPCR or cDNA-library workflows. |
| Sample Type | Animal, plant and viral RNA, including preparations containing ethanol, isopropanol, water-saturated phenol or humic acid. |
| Performance Range / Specifications | 10,000 U; efficient reverse transcription at 37°C, with optional 50–55°C reactions for structured RNA templates. |
| Sensitivity / LOD | High reverse-transcription efficiency supports downstream detection of challenging RNA targets. |
| Specificity | Elevated-temperature operation reduces secondary-structure interference for complex RNA templates. |
| Reaction Conditions / Protocol | Use with a compatible reverse-transcription buffer, dNTPs, RNase inhibitor and random, oligo(dT) or gene-specific primers. Select 37°C for routine templates or 50–55°C for highly structured RNA. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 10,000 U. |
| Product Form | High-efficiency reverse transcriptase enzyme solution. |
| Quality Control | Template compatibility, contaminant tolerance, reverse-transcription efficiency and long-target performance are evaluated. |
| Key Features | Broad animal, plant and viral RNA compatibility; strong tolerance to common contaminants; efficient cDNA synthesis; high processivity; elevated-temperature operation. |
| Compatibility | Suitable for first-strand cDNA synthesis, two-step RT-qPCR and cDNA-library construction. |
| Application Notes / Precautions | RNA quality should be verified before reverse transcription. Use random primers for broad RNA coverage and oligo(dT) or gene-specific primers when long transcript representation is required. |
For research use only, not for clinical use.
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