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| Product Name | Green Fluorescent Cadaverine Probe, Carboxyl-Reactive (Ex/Em 496/519 nm) |
| Catalog No. | RERE-0005 |
| Description | This cadaverine-based dye carries a carbonyl-reactive structural unit: in the presence of an activator or an activated ester, its amine group modifies carboxyl groups through stable amide bonds. The signal it produces is bright and resistant to photobleaching, so it stands up to extended fluorescence observation. Typical use is the labelling of cells and biological molecules through their carboxyl chemistry. |
| Intended Use | Carboxyl-group modification and labelling of cells and biological molecules under activator or activated-ester conditions. |
| Principle / Technology | The cadaverine arm provides primary amines that react with activated carboxyl groups, giving stable amide bonds; the fluorescein-type core then contributes a bright, photostable green signal. |
| Detection Method | Fluorescence detection with excitation/emission of 496/519 nm. |
| Sample Type | Orange-yellow solid. |
| Performance Range / Specifications | Molecular formula C26H27N4O10S2+; molecular weight 619.12 g/mol; appearance orange-yellow solid; purity ≥95%; Ex/Em 496/519 nm; reactive unit: carbonyl-reactive amine. |
| Specificity | Carbonyl (carboxyl)-reactive rather than amine-reactive |
| Reaction Conditions / Protocol | In the presence of a carboxyl activator or a pre-formed activated ester, react the probe with the carboxyl-bearing substrate, then purify the labelled product. |
| Components / Formulation | One vial of dye; activators and solvents are supplied by the user. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 1 mg |
| Product Form | Orange-yellow solid. |
| Quality Control | Released at purity ≥95%. |
| Key Features | - Carbonyl-reactive chemistry targets carboxyl groups - Bright signal with strong photostability for long observations - Purity released at ≥95% - Stable amide-bond labelling under mild conditions - One-year shelf life protected from light |
| Purity | ≥95% |
| Activity / Unit Definition | No unit definition; defined by spectral brightness and photostability. |
| Molecular Weight | Molecular formula C26H27N4O10S2+; molecular weight 619.12 g/mol |
| Source / Origin | Synthetic small-molecule fluorophore, supplied as a purified solid. |
| Expiration Date / Stability | Remains stable for the stated period provided the cold chain is not broken and light exposure is avoided. |
| Regulatory / Compliance | Supplied for laboratory research purposes. |
| Compatibility | Works on common fluorescence platforms; match the filter set to the excitation/emission values listed for this product. |
| Recommended Buffer System | No buffer is included; prepare dry stock shortly before the coupling step. |
| Application Notes / Precautions | Wear gloves when handling DMSO stocks, keep vials in the dark, and let chilled vials warm to room temperature before opening to avoid condensation. |
| Batch-to-Batch Consistency | Consistency between lots is maintained by fixed production controls and release testing against specification. |
| Model Type | Carboxyl-reactive fluorescent probe (cadaverine chemistry) |
| Starting Material / Tissue Site | Synthesised fluorescein-type fluorophore bearing a cadaverine reactive arm |
| Delivery Standard | Pack size 1 mg |
| Turnaround Time | dispatch timing is settled at purchase. |
| Readouts / Downstream Assays | Excitation/emission 496/519 nm on a green channel. |
For research use only, not for clinical use.
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