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| Product Name | Gram Stain Kit, Four-Reagent Set, for Bacterial Classification |
| Catalog No. | ATRSA-0008 |
| Description | A complete Gram staining kit containing crystal violet, Gram's iodine, decolorizer, and safranin counterstain for the differential classification of bacteria into Gram-positive and Gram-negative groups by light microscopy. |
| Intended Use | Differential staining of bacterial smears for Gram classification in clinical microbiology, pharmaceutical quality control, environmental monitoring, and general bacteriology teaching laboratories. |
| Principle / Technology | Crystal violet stains all bacterial cells; iodine forms crystal violet-iodine complex; decolorizer removes complex from thin peptidoglycan layer of Gram-negative bacteria; safranin counterstains decolorized Gram-negative cells pink. |
| Detection Method | Bright-field light microscopy at 100x oil immersion |
| Sample Type | Bacterial colonies from culture plates, clinical specimens, broth cultures, environmental swabs |
| Performance Range / Specifications | Correct classification of ATCC reference strains: S. aureus (Gram+) and E. coli (Gram-) |
| Sensitivity / LOD | N/A |
| Specificity | Distinguishes Gram-positive (purple) from Gram-negative (pink) bacteria reliably for most clinically relevant species |
| Reaction Conditions / Protocol | Heat-fix bacterial smear; crystal violet 1 min; rinse; iodine 1 min; rinse; decolorize dropwise until runoff clear; rinse; safranin 30 sec–1 min; rinse; blot dry. |
| Components / Formulation | Crystal Violet solution, Gram's Iodine (iodine-potassium iodide), Decolorizer (acetone-ethanol), Safranin O counterstain |
| Storage Conditions | 15–30°C, tightly sealed, protect from light, flammable components |
| Shelf Life | 24 months |
| Package Specifications | Kit for 250 tests, 500 tests |
| Product Form | Liquid reagents in dropper bottles |
| Quality Control | Gram reaction verification with ATCC 25923 (S. aureus) and ATCC 25922 (E. coli), staining intensity reproducibility |
| Key Features | Complete kit; ready to use; validated with ATCC strains; standard for clinical microbiology; dropper bottles for convenience |
For research use only, not for clinical use.
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