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| Product Name | Giemsa Stain Solution, Modified, for Malaria and Blood Parasites |
| Catalog No. | ATRSA-0001 |
| Description | A high-quality Giemsa azure-eosin-methylene blue staining solution specifically formulated for the microscopic detection and differentiation of malaria parasites, blood parasites, and chromosomal banding patterns. |
| Intended Use | Microscopic examination of thick and thin blood films for malaria diagnosis (Plasmodium species identification), Babesia, Trypanosoma, and Leishmania detection; chromosome G-banding in cytogenetics. |
| Principle / Technology | Azure-eosinate and methylene blue-eosinate complexes differentially stain acidic (basophilic) structures blue-purple and basic (eosinophilic) structures pink-red, revealing nuclear and cytoplasmic details of parasites with distinctive magenta chromatin. |
| Detection Method | Light microscopy at 100x oil immersion for malaria diagnosis; Giemsa banding for metaphase chromosomes |
| Sample Type | Thin and thick blood films, bone marrow aspirates, chromosomal preparations, tissue touch preparations |
| Performance Range / Specifications | Plasmodium species discrimination sensitivity at 5–10 parasites/µL in thick film examination |
| Sensitivity / LOD | Detects 5–10 parasites per microliter in thick film examination by experienced microscopist |
| Specificity | Distinguishes Plasmodium falciparum, P. vivax, P. ovale, P. malariae based on morphology and Schüffner dots |
| Reaction Conditions / Protocol | Fix thin film in methanol for 30 sec; stain in 1:20 working dilution of Giemsa for 20–30 min; rinse with buffered water pH 7.2; air dry. |
| Components / Formulation | Azure II-eosin, Azure II, Methylene Blue-eosin, Methylene Blue chloride in glycerol-methanol vehicle |
| Storage Conditions | 15–30°C, tightly sealed, protect from light and moisture |
| Shelf Life | 36 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid stain concentrate |
| Quality Control | Staining intensity and differentiation testing with Plasmodium-positive blood film reference slides |
| Key Features | Stable stock solution; distinctive Romanowsky staining pattern; validated for malaria diagnosis; buffered water mounting recommended |
For research use only, not for clinical use.
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