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| Product Name | Glycosylated Serum Protein (GSP) Content Assay Reagent (Visible Spectrophotometer) |
| Catalog No. | PTR-HMM-0047 |
| Description | Glycated serum protein (GSP) is a high-molecular-weight ketamine-like substance formed during the non-enzymatic glycation of plasma proteins with glucose. Its concentration is positively correlated with blood glucose levels and remains relatively stable, reflecting the average blood glucose level over the past 1–3 weeks. It serves as an important indicator for distinguishing stress-induced hyperglycemia and evaluating diabetes control. |
| Application | Serum glucose undergoes non-enzymatic glycation reactions with the N-terminal amino groups of albumin and other serum proteins, forming high-molecular-weight ketamine structures. Under alkaline conditions, these react with nitro-tetrachlorozon blue to form the purple-red compound methanethiol, which has a characteristic absorption peak at 530 nm. The concentration of glycated serum proteins can be quantitatively determined by measuring changes in absorbance. |
| Applicable Instruments | Visible Spectrophotometer |
| Number of Testable Samples | 48 Samples |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Detection Time | 2 h (48 Samples) |
| Detection Method | NBT |
| Spectral Parameters | 530 nm |
| Signal Response | Incremental |
| Notes | After color development at 37°C is complete, reagent four should be added immediately; it is recommended not to process too many samples at once to prevent delayed addition of reagent four from affecting the measurement results; If the A measurement is greater than 1.0 or the ΔA measurement is greater than 0.8, it is recommended to dilute the sample appropriately before remeasuring, and multiply the dilution factor in the calculation formula; If the measured absorbance value is low or接近 the blank value, it is recommended to increase the sample volume during sample preparation, re-extract, and then remeasure, making corresponding adjustments in the calculation; |
For research use only, not for clinical use.
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