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| Product Name | FluoroProfile Protein Quantification Kit (Fluorometric, 0.02-2 µg/mL) |
| Catalog No. | PTR-HMM-0089 |
| Description | Ultrasensitive fluorometric protein quantification kit using a proprietary fluorogenic dye that fluoresces upon non-covalent binding to protein hydrophobic regions. With a detection range of 0.02-2 µg/mL, this assay is 250-fold more sensitive than standard BCA and 10-fold more sensitive than Micro BCA. Designed for quantifying extremely dilute protein samples from laser capture microdissection, single-cell proteomics preparations, and trace protein analysis. |
| Intended Use | Ultrasensitive protein quantification of dilute samples; luminescence-based detection complement; laser capture microdissection (LCM) sample quantification; single-cell proteomics; trace protein determination in process impurities; protein quantification for ultrasensitive ELISA. |
| Principle / Technology | Proprietary fluorogenic dye is essentially non-fluorescent in aqueous solution but fluoresces strongly (Ex/Em 485/530 nm) upon binding to hydrophobic regions of proteins; fluorescence intensity is proportional to protein concentration; minimal protein-to-protein variation due to broad hydrophobic region recognition |
| Detection Method | Fluorometric; excitation 480-485 nm, emission 525-535 nm; 96-well or 384-well black plate format |
| Sample Type | Aqueous protein solutions; cell/tissue lysates after high dilution; immunoprecipitation eluates; column fractions; samples with 0.02-2 µg/mL protein range |
| Sensitivity / LOD | Detection 0.02-2 µg/mL BSA (250× more sensitive than BCA); linear dynamic range 2 orders of magnitude; 0.02 µg/mL is detectable above blank |
| Specificity | Binds hydrophobic protein regions; less protein-to-protein variation than Coomassie dye-binding; free amino acids, peptides <3 kDa, and nucleic acids show minimal interference |
| Reaction Conditions / Protocol | Add 10-100 µL dilute protein sample to black plate; add equal volume of FluoroProfile working reagent; mix gently; incubate 5-15 min at RT protected from light; read fluorescence Ex/Em 485/530 nm; compare to BSA standard curve |
| Components / Formulation | FluoroProfile dye reagent (200× concentrate in DMSO), BSA protein standard (2 mg/mL), assay buffer (PBS pH 7.4), black 96-well plate (optional), adhesive plate seal |
| Storage Conditions | 2-8 °C for dye reagent; -20 °C for long-term; protect from light; BSA standard at 4 °C |
| Shelf Life | 12 months at -20 °C; 6 months at 4 °C; working reagent stable 2 hr at RT, 24 hr at 4 °C |
| Package Specifications | 200 assays, 1,000 assays (96-well); 4,000 assays (384-well) |
| Product Form | Liquid dye concentrate in DMSO; BSA standard in PBS |
| Key Features | Ultrasensitive detection to 0.02 µg/mL; 250× more sensitive than BCA; rapid 5-15 min incubation; broad protein specificity; compatible with detergents at working concentrations; suitable for high-throughput screening; fluorescence plate reader required |
| Purity | Dye purity >97% by HPLC; BSA standard >98%; no detectable protein contamination in dye reagent |
| Concentration | As specified on product label or certificate of analysis |
| Activity / Unit Definition | Binding capacity or enzymatic activity as specified per product documentation |
| Molecular Weight | Varies by product as specified in documentation |
| Source / Origin | Recombinant expression in E. coli, mammalian, or insect cell systems; synthetic chemistry for small molecule reagents |
| pH Range / Optimal pH | pH 7.0–8.0 for most protein applications |
| Shipping Conditions | Cold pack 2-8 °C; protect from light; dye reagent in DMSO — flammable liquid regulations apply |
| Expiration Date / Stability | 12 months at -20 °C; 6 months at 4 °C; DMSO stock stable at -20 °C; discard if dye reagent develops color or fluorescence in absence of protein (indicates degradation) |
| Regulatory / Compliance | For laboratory and research use only; RUO; dye in DMSO — flammable; ISO 9001 |
| Compatibility | Compatible with standard SDS-PAGE, Western blot, mass spectrometry, and chromatography systems |
| Recommended Buffer System | PBS, Tris, or HEPES buffers as specified in product protocol |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles for protein solutions; use fresh aliquots; protect light-sensitive reagents |
| Batch-to-Batch Consistency | Protein concentration and activity within ±15% of reference lot |
For research use only, not for clinical use.
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