- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Fluor 680 Phalloidin |
| Catalog No. | RESK-0578 |
| Description | Fluor 680 Phalloidin is a far-red fluorescent conjugate of phalloidin, a cyclic heptapeptide toxin derived from the mushroom Amanita phalloides. Phalloidin selectively binds filamentous actin (F-actin) with high affinity (Kd = 20 nM) and does not bind monomeric actin (G-actin), making it a standard tool for labeling F-actin in tissue sections, cell cultures, and cell-free systems for qualitative and quantitative analysis. Phalloidin conjugates bind large and small actin filaments in muscle and non-muscle cells of both animal and plant origin with approximately one phalloidin molecule per actin subunit, and nonspecific binding is negligible, providing exceptionally sharp contrast between stained and unstained regions. Phalloidin derivatives are very small (about 12-15 Å in diameter, molecular weight < 2000 Da), so many physiological properties of the labeled actin are preserved: actin-binding proteins such as myosin, tropomyosin, and DNase I can still interact with labeled filaments, phalloidin-labeled filaments can still penetrate solid-phase myosin matrices, and glycerol-extracted muscle fibers remain contractile after labeling. Phalloidin binding also prevents the dissociation of F-actin (microfilaments), stabilizes microfilament structure, and disrupts the polymerization-depolymerization equilibrium, lowering the critical concentration (CC) of actin polymerization to < 1 µg/mL; phalloidin therefore also acts as a polymerization promoter and inhibits the ATP-hydrolyzing activity of F-actin. Fluor 680 Phalloidin delivers highly specific staining with strong contrast, outperforming actin antibodies for qualitative and quantitative F-actin detection. Fluor 680 Phalloidin staining is fully compatible with other fluorescent labels used in cell analysis, including fluorescent proteins, semiconductor nanocrystals, and other Fluor dye conjugates (including Fluor-conjugated secondary antibodies). F-actin bound by this product retains many of the native biological properties of actin, and binding shows no species specificity, ensuring broad applicability. The product is supplied as powder pellets; reconstitute with 30 µL DMSO and mix thoroughly before use. |
| Intended Use | For fluorescent labeling of filamentous actin (F-actin) in tissue sections, cultured cells, and cell-free systems, enabling qualitative and quantitative analysis of the actin cytoskeleton. |
| Principle / Technology | Phalloidin is a cyclic heptapeptide toxin from Amanita phalloides that binds F-actin selectively and with high affinity (Kd = 20 nM) without binding G-actin. Conjugation to the far-red fluorescent dye Fluor 680 allows direct visualization of F-actin. Binding stabilizes microfilaments by blocking their dissociation, lowers the critical concentration of actin polymerization to < 1 µg/mL, and inhibits F-actin ATP hydrolysis. |
| Detection Method | Fluorescence microscopy / fluorescence imaging |
| Sample Type | Tissue sections, cultured cells, and cell-free systems; muscle and non-muscle cells of animal or plant origin |
| Performance Range / Specifications | Size: 300 T Maximum excitation/emission (Ex/Em): 684/701 nm Molecular weight: ~2000 Solubility: soluble in DMSO |
| Sensitivity / LOD | High-affinity binding to F-actin with Kd = 20 nM; nonspecific binding is negligible, giving clearly distinguishable stained and unstained regions. |
| Specificity | Selectively binds filamentous actin (F-actin); does not bind monomeric actin (G-actin). No species-dependent differences in binding. |
| Reaction Conditions / Protocol | Supplied as powder pellets. Add 30 µL DMSO and mix thoroughly to reconstitute before staining. |
| Components / Formulation | Fluor 680 Phalloidin (Fluor 680-conjugated phalloidin), supplied as powder pellets; soluble in DMSO. |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 1 year |
| Package Specifications | 300 T |
| Product Form | Powder pellets |
| Molecular Weight | ~2000 |
| Source / Origin | Phalloidin derived from the mushroom Amanita phalloides |
| Shipping Conditions | Shipped on ice packs. |
| Expiration Date / Stability | Valid for 1 year when stored at -25 to -15 °C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Fully compatible with other fluorescent stains used in cell analysis, including fluorescent proteins, semiconductor nanocrystals, and other Fluor dye conjugates (including Fluor-conjugated secondary antibodies). F-actin bound by this product retains many native biological properties of actin. Binding is species-independent. |
| Recognition Site | Filamentous actin (F-actin); binds approximately one phalloidin molecule per actin subunit |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
