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| Product Name | Fluor 488 NHS Ester |
| Catalog No. | RESK-0569 |
| Description | Fluor 488 is a bright green fluorescent dye with water solubility and strong pH tolerance (pH 4-10). It can be excited at 488 nm to produce a stable fluorescent signal and is commonly used as a cell labeling reagent conjugated to antibodies, peptides, proteins, tracers, and other substrates, with broad application in cell imaging and detection. Fluor 488 NHS Ester carries an N-hydroxysuccinimide (NHS) reactive group and reacts directly with amino groups (-NH2), primarily primary amines, on biomolecules to form stable amide bonds. It can be used to label any protein or peptide containing primary amines, amino-modified oligonucleotides, or other amine-containing biomolecules. |
| Intended Use | Fluorescent labeling of antibodies, peptides, proteins, tracers, amino-modified oligonucleotides, and other amine-containing biomolecules for cell imaging and detection. |
| Principle / Technology | The N-hydroxysuccinimide (NHS) ester reactive group reacts directly with amino groups (-NH2), primarily primary amines, on biomolecules to form stable amide bonds, covalently attaching the Fluor 488 green fluorophore. |
| Detection Method | Green fluorescence detection with excitation at 488 nm (Ex/Em 494/517 nm) for cell imaging and detection. |
| Sample Type | Antibodies, proteins, peptides, amino-modified oligonucleotides, and other biomolecules containing primary amines. |
| Performance Range / Specifications | Fluorophore Label: Fluor 488 dye Reactive Group: NHS ester Reactivity: primary amines (R-NH2) Molecular Formula: C25H15Li2N3O13S2 Molecular Weight: 643.4 Appearance: powder Ex/Em: 494/517 nm Extinction Coefficient: 71,000 cm-1 M-1 Correction Factor (CF280): 0.11 |
| Specificity | Reacts with amino groups (-NH2), primarily primary amines, forming stable amide bonds. |
| Reaction Conditions / Protocol | The NHS ester reacts directly with primary amines on the target biomolecule to form stable amide bonds; suitable for labeling proteins, peptides, amino-modified oligonucleotides, and other amine-containing biomolecules. |
| Components / Formulation | Fluor 488 NHS ester (succinimidyl ester). |
| Storage Conditions | Store at -20 °C, dry and protected from light. |
| Shelf Life | 6 months when stored as recommended. |
| Package Specifications | 1 mg |
| Product Form | Powder. |
| Molecular Weight | 643.4 |
| pH Range / Optimal pH | Strong pH tolerance over pH 4-10. |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for 6 months when stored at -20 °C, dry and protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Excited at 488 nm, compatible with standard 488 nm laser lines used in cell imaging and detection platforms; water-soluble dye. |
| Application Notes / Precautions | Water-soluble dye with strong pH tolerance (pH 4-10) and stable fluorescent signal. Protect from light during storage and handling. |
For research use only, not for clinical use.
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