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| Product Name | FLAG Peptide Competitive Elution Reagent |
| Catalog No. | PTR-HMM-0071 |
| Description | A 3×FLAG peptide solution for the gentle competitive elution of FLAG-tagged recombinant proteins from anti-FLAG affinity resins. This elution method preserves protein activity by avoiding the harsh pH conditions used in conventional elution protocols. |
| Intended Use | Gentle, non-denaturing elution of FLAG-tagged fusion proteins from anti-FLAG affinity purification resins, maintaining native protein conformation and biological activity. |
| Principle / Technology | Synthetic 3×FLAG peptide (DYKDHDGDYKDHDIDYKDDDDK) competes with the FLAG epitope tag on the fusion protein for binding to the immobilized anti-FLAG monoclonal antibody, resulting in specific displacement and elution of the tagged protein. |
| Detection Method | Elution under physiological pH and ionic strength conditions; eluted protein is recovered in a biologically active state |
| Sample Type | FLAG-tagged recombinant proteins bound to anti-FLAG M2 or other FLAG-specific affinity resins |
| Performance Range / Specifications | Effective elution achieved with 100–150 μg/mL 3×FLAG peptide in TBS buffer; typical elution volume is 2–5 column bed volumes |
| Sensitivity / LOD | Complete elution of FLAG-tagged proteins at concentrations as low as 50 μg/mL |
| Specificity | The 3×FLAG peptide sequence is specifically recognized by anti-FLAG monoclonal antibodies; it does not cross-react with other epitope tag systems (HA, Myc, V5, His) |
| Reaction Conditions / Protocol | After binding FLAG-tagged protein to the anti-FLAG resin and washing, apply 3×FLAG peptide solution (150 μg/mL in TBS), incubate for 30 minutes at 4°C with gentle agitation, collect eluate, repeat if needed for complete recovery |
| Components / Formulation | Lyophilized 3×FLAG peptide (sufficient for preparation of 50 mL of elution buffer), TBS buffer salts for elution buffer preparation |
| Storage Conditions | Lyophilized peptide at -20°C desiccated; reconstituted peptide solution can be stored at 2–8°C for 2 weeks or aliquoted and frozen at -20°C for 3 months |
| Shelf Life | 24 months for lyophilized peptide |
| Package Specifications | 5 mg of lyophilized 3×FLAG peptide |
| Product Form | White lyophilized powder |
| Quality Control | Peptide purity >95% by HPLC; molecular weight confirmed by mass spectrometry; biological activity verified by competitive elution of FLAG-tagged protein standard from anti-FLAG resin |
| Key Features | Gentle elution preserves protein activity and complex integrity for downstream functional assays; no residual peptide contamination when followed by dialysis or desalting; compatible with tandem affinity purification strategies |
For research use only, not for clinical use.
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