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| Product Name | FBS-Based Cell Culture Specimen Diluent |
| Catalog No. | ATRD-0007 |
| Description | A fetal bovine serum-based diluent providing optimal protein and growth factor supplementation for the dilution and maintenance of primary cells, cell culture supernatants, and virus stocks. |
| Intended Use | Diluting cell suspensions, viral stocks, and cell culture-derived analytes while maintaining cell viability and biomolecule stability during experimental manipulations. |
| Principle / Technology | FBS provides growth factors, attachment factors, and protective proteins that maintain cell membrane integrity and protein stability during dilution procedures in low serum conditions. |
| Detection Method | Cell viability by trypan blue exclusion, viral titer comparison pre/post dilution |
| Sample Type | Primary cells, established cell lines, virus stocks, cell culture supernatants |
| Performance Range / Specifications | Cell viability >90% after 4-hour incubation in diluent at 4°C or 37°C |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add appropriate volume to cell pellet or concentrated stock; mix gently; use within time frame validated for specific cell type. |
| Components / Formulation | DMEM or RPMI base, 10% heat-inactivated FBS, 1% penicillin-streptomycin, 25 mM HEPES |
| Storage Conditions | 2–8°C; warm to 37°C before use |
| Shelf Life | 3 months when stored sterile |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Sterility, endotoxin <0.5 EU/mL, FBS lot consistency testing for growth promotion |
| Key Features | Cell culture grade; sterile; serum-supplemented; maintains cell viability during processing; validated with common cell lines |
For research use only, not for clinical use.
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