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| Product Name | Exosome Red Fluorescent Tracking Kit (PKH26) |
| Catalog No. | RESK-0570 |
| Description | Exosomes are small vesicles of approximately 30-150 nm in diameter secreted by living cells, with a typical lipid bilayer structure. They are present in cell culture supernatants, serum, plasma, saliva, urine, amniotic fluid and other biological fluids. Exosomes carry important cargo such as proteins, lipids, DNA and RNA; they not only play a key role in intercellular material and information transfer, but are also promising early diagnostic biomarkers for a variety of diseases. This kit uses PKH26, a red fluorescent dye with a lipid tail, to label the lipid regions of exosomal membranes. The kit increases staining efficiency during the labeling procedure while maintaining cell viability. It is suitable for exosome labeling and staining experiments and for monitoring cellular phagocytosis. |
| Intended Use | For fluorescent labeling of exosomes and monitoring of exosome uptake and cellular phagocytosis in research applications. |
| Principle / Technology | PKH26 is a red fluorescent dye with a lipid tail that incorporates into the lipid regions of cell and exosomal membranes. Upon incorporation, the labeled exosomes exhibit strong red fluorescence that can be followed by fluorescence-based detection methods. |
| Detection Method | Fluorescence detection (red fluorescence, Ex/Em 551/567 nm). |
| Sample Type | Isolated exosomes from cell culture supernatant, serum, plasma, saliva, urine, amniotic fluid and other biological fluids. |
| Performance Range / Specifications | Chemical name: PKH26 CAS NO.: 154214-55-8 Formula: C59H97IN2 Molecular weight: 961.32 Appearance: liquid Excitation wavelength (Ex): 551 nm Emission wavelength (Em): 567 nm |
| Reaction Conditions / Protocol | 1. Exosome protein quantification: take an appropriate amount of exosomes and determine the exosomal protein amount by BCA protein assay. 2. Preparation of dye working solution: dilute the PKH26 stock solution 10-fold with Dilution buffer to prepare a 100 μM dye working solution (protect from light; prepare the working solution fresh according to the amount required and use it immediately). 3. Exosome staining: (1) Add the dye working solution to the exosomes. A final dye concentration of 5 μM is recommended, added at a dye working solution to exosome volume ratio of 1:19 (the final concentration can be adjusted within 2-20 μM). (2) After adding the dye working solution, close the centrifuge tube tightly, mix on a vortex mixer for 1 min, then incubate statically for 10 min. (3) Add 10 mL of 1x PBS to the incubated exosome-dye complex and mix well. (4) Re-isolate the exosomes according to the exosome extraction method to remove excess dye. (5) Resuspend the pellet in 200 μL of 1x PBS; the pellet is the stained exosomes. |
| Components / Formulation | PKH26 dye stock solution Dilution buffer |
| Storage Conditions | Upon receipt, store the reagents separately and mix thoroughly before use. Store at 2-8 °C protected from light. |
| Shelf Life | 12 months when stored at 2-8 °C protected from light. |
| Package Specifications | 20 μL |
| Product Form | Liquid |
| Concentration | Dye working solution: 100 μM (prepared by 10-fold dilution of the stock solution); recommended final staining concentration: 5 μM (adjustable within 2-20 μM). |
| Molecular Weight | 961.32 |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for 12 months when stored at 2-8 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Recommended Buffer System | 1x PBS |
| Application Notes / Precautions | 1. The PKH26 stock solution is prone to hydrolysis; aliquoting is recommended, and aliquots should be sealed with parafilm for storage. 2. The PKH26 working solution should be prepared fresh and used immediately; do not prepare it in advance, otherwise the staining effect will be compromised. 3. The PKH26 diluent may solidify and stick to the bottom of the tube at low temperature; warm briefly in a 37 °C water bath until fully dissolved before use. 4. For your safety and health, wear a lab coat and disposable gloves during operation. 5. Over-staining can cause loss of exosomal membrane integrity; the optimal PKH26 dye to exosome ratio should be determined empirically for each experiment. 6. The reagents in this kit are not sterile; if sterility is required, sterilize by filtration. 7. If isolated exosomes are to be stored before staining, store them at -80 °C. |
For research use only, not for clinical use.
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