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| Product Name | Ethidium Bromide Solution, 10 mg/mL, Molecular Biology Grade |
| Catalog No. | ATRSA-0006 |
| Description | A concentrated ethidium bromide solution at 10 mg/mL for use as a fluorescent nucleic acid intercalating stain in agarose gel electrophoresis, with well-established sensitivity and staining characteristics. |
| Intended Use | Fluorescent detection of DNA in agarose gels following electrophoresis for molecular biology research applications in laboratories with appropriate chemical safety protocols. |
| Principle / Technology | Ethidium bromide (3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide) intercalates between DNA base pairs with a 20–25 fold fluorescence enhancement upon binding; excitation maximum at 300–360 nm, emission at 590 nm. |
| Detection Method | UV transillumination at 302 nm, fluorescence imaging with ethidium bromide filter set |
| Sample Type | PCR products, restriction enzyme digests, plasmid DNA, genomic DNA in agarose gels |
| Performance Range / Specifications | Detection limit ~1–2 ng DNA per band; linear signal ~5–500 ng per band |
| Sensitivity / LOD | 1–2 ng per band for double-stranded DNA under optimal UV excitation |
| Specificity | Binds both double-stranded DNA and RNA; does not detect single-stranded nucleic acids efficiently |
| Reaction Conditions / Protocol | Add to molten agarose at 0.5 µg/mL final concentration before gel pouring; or post-stain gel in 0.5 µg/mL solution for 15–30 min with gentle agitation. |
| Components / Formulation | Ethidium bromide 10 mg/mL in ultrapure water, passed through 0.22 µm filter |
| Storage Conditions | 15–30°C, protect from light; mutagen: handle with appropriate PPE |
| Shelf Life | 36 months |
| Package Specifications | 10 mL dropper bottle |
| Product Form | Liquid concentrate |
| Quality Control | Absorbance spectrum verification, concentration by spectrophotometry, DNase/RNase free |
| Key Features | Well-established staining characteristics; economical; high concentration for custom dilution; validated for DNA visualization |
For research use only, not for clinical use.
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